Molecular species of ethanolamine phosphatides of dog and pig kidney.
Molecular species of ethanolamine phosphatides of dog and pig kidney.
复制标题
狗和猪肾的乙醇胺磷脂的分子种类。
DOI：
10.1139/o74-119
复制
发表时间：
1974
期刊：
影响因子：
--
通讯作者：
A. Kuksis
中科院分区：
文献类型：
--
作者：
S. K. Yeung;A. Kuksis
用薄层色谱法分离了狗肾和猪肾的乙醇胺总磷脂。（薄层色谱法）和通过转甲基化衍生的脂肪酸甲酯和二甲基缩醛通过气-液色谱法和质谱法相结合的方法鉴定。结果表明，40-50%的乙醇胺磷脂从这两个来源是piasmalogens其中长链乙烯基醚主要与花生四烯酸配对。虽然猪肾的乙烯基醚主要产生棕榈醛，但狗肾的乙烯基醚产生几乎相等比例的棕榈醛、硬脂醛和油醛。没有证据表明这些磷脂馏分中存在乙醇胺以外的含氮碱。指出了上述观察结果与肾磷脂潜在抗肾素活性有关的意义。
The total ethanolamine phosphatides of the dog and pig kidney were isolated by T.L.C. (thin-layer chromatography) and the fatty acid methyl esters and dimethylacetals derived by transmethylation were identified by combined gas–liquid chromatography and mass spectrometry methods. It was shown that 40–50% of the ethanolamine phosphatides from both sources were piasmalogens in which the long-chain vinyl ethers were paired largely with arachidonic acid. Although the vinyl ethers of the pig kidney yielded mainly the palmitaldehyde, those of the dog kidney generated nearly equal proportions of palmit-, stear-, and oleoaldehydes. No evidence was obtained for the occurrence of nitrogenous bases other than ethanolamine in these phosphatide fractions. The significance of the above observations in relation to the potential anti-renin activity of the kidney phospholipids is pointed out.