Graphene Fluorescence Resonance Energy Transfer Aptasensor for the Thrombin Detection
Graphene Fluorescence Resonance Energy Transfer Aptasensor for the Thrombin Detection
复制标题
用于凝血酶检测的石墨烯荧光共振能量转移适体传感器
DOI：
10.1021/ac9025384
复制
发表时间：
2010-03-15
影响因子：
7.4
通讯作者：
Li, Jinghong
中科院分区：
文献类型：
--
作者：
Chang, Haixin;Tang, Longhua;Li, Jinghong
将纳米材料与生物分子识别单元相结合，有望开发新的临床诊断和蛋白质分析技术。在这项工作中，基于染料标记的适体组装石墨烯，开发了一种用于凝血酶检测的高灵敏度和特异性荧光共振能量转移（FRET）适体传感器。由于适配体和石墨烯之间的非共价组装，由于FRET，染料的荧光猝灭发生。凝血酶的加入导致荧光恢复，这是由于四复体凝血酶复合物对石墨烯具有弱亲和力，并使染料远离石墨烯表面。由于石墨烯的高荧光猝灭效率、独特的结构和电子特性，石墨烯适体传感器在缓冲液和血清中都表现出极高的灵敏度和良好的特异性。基于石墨烯FRET适体传感器的检测限低至31.3 pM，比基于碳纳米管的荧光传感器低两个数量级。基于石墨烯的FRET感光器的优异性能也将归因于石墨烯独特的结构和电子特性。
Combining nanomaterials and biomolecule recognition units is promising in developing novel clinic diagnostic and protein analysis techniques. In this work, a highly sensitive and specific fluorescence resonance energy transfer (FRET) aptasensor for thrombin detection is developed based on the dye labeled aptamer assembled graphene. Due to the noncovalent assembly between aptamer and graphene, fluorescence quenching of the dye takes place because of FRET. The addition of thrombin leads to the fluorescence recovery due to the formation of quadruplex-thrombin complexes which have weak affinity to graphene and keep the dyes away from graphene surface. Because of the high fluorescence quenching efficiency, unique structure, and electronic properties of graphene, the graphene aptasensor exhibits extraordinarily high sensitivity and excellent specificity in both buffer and blood serum. A detection limit as low as 31.3 pM is obtained based on the graphene FRET aptasensor, which is two orders magnitude lower than those of fluorescent sensors based on carbon nanotubes. The excellent performance of FRET aptasensor based on graphene will also be ascribed to the unique structure and electronic properties of graphene.