Hormone-controlled cAMP-mediated fluid secretion in yellow-fever mosquito.
Hormone-controlled cAMP-mediated fluid secretion in yellow-fever mosquito.
复制标题
黄热病蚊子中激素控制的 cAMP 介导的液体分泌。
DOI：
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发表时间：
1987
影响因子：
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通讯作者：
K. Beyenbach
中科院分区：
文献类型：
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作者：
D. Petzel;M. M. Berg;K. Beyenbach
证据表明，在吸血黄热病蚊子埃及伊蚊的马氏管中，存在由腺苷控制的环磷酸腺苷（cAMP）介导的NaCl利尿作用。在离体马氏管中的研究表明，cAMP添加到管周浴选择性刺激NaCl分泌，而不是KCl分泌，通过增加Na电导的基底外侧膜的原代细胞。这些效果是复制的毛喉素和茶碱在平行增加内源性cAMP的细胞内浓度。两种利钠肽，我们已经分离出的高压液相色谱法（HPLC）的方法从蚊子头也增加氯化钠和液体分泌在孤立的马氏管连同增加细胞内cAMP水平。这些结果与NaCl利尿的机制一致，其中利钠肽和cAMP分别是将血餐的摄入与餐中不需要的盐和水部分的排泄相结合的主要和次要信使。这一假设得到了体内研究的支持，这些研究揭示了在最大NaCl利尿时马氏管中细胞内cAMP水平升高。
Evidence is presented for hormone-controlled adenosine 3',5'-cyclic monophosphate (cAMP)-mediated NaCl diuresis in Malpighian tubules of the blood-feeding yellow-fever mosquito Aedes aegypti. Studies in isolated Malpighian tubules reveal that cAMP added to the peritubular bath selectively stimulates NaCl secretion and not KCl secretion by increasing the Na conductance of the basolateral membrane of primary cells. These effects are duplicated by forskolin and theophylline in parallel with increased intracellular concentrations of endogenous cAMP. Two natriuretic peptides that we have isolated by high-pressure liquid chromatography (HPLC) methods from mosquito heads also increase NaCl and fluid secretion in isolated Malpighian tubules together with increased intracellular levels of cAMP. These results are consistent with a mechanism of NaCl diuresis in which the natriuretic peptides and cAMP are respectively the primary and secondary messengers that couple the ingestion of a blood meal to the excretion of the unwanted salt and water fraction of the meal. This hypothesis is supported by in vivo studies that reveal elevated intracellular cAMP levels in Malpighian tubules at the time of maximum NaCl diuresis.