An in situ cross-linking hybrid hydrogel for controlled release of proteins.
An in situ cross-linking hybrid hydrogel for controlled release of proteins.
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DOI：
10.1016/j.actbio.2012.01.028
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发表时间：
2012-05
影响因子：
9.7
通讯作者：
Kohane, Daniel S.
中科院分区：
文献类型：
--
作者：
Epstein-Barash, Hila;Stefanescu, Cristina F.;Kohane, Daniel S.
显然需要提供治疗性蛋白质的安全控释的方法，以实现和维持高的局部蛋白质浓度，或用于持续的全身递送。在这里，我们已经开发了一种蛋白质递送系统结合原位交联多糖水凝胶与明胶。该制剂是可注射的，易于应用，并且在制剂过程中避免了对有机溶剂或潜在有毒交联剂的需要。交联多糖本身（包括透明质酸、葡聚糖和/或羧甲基纤维素）提供荧光标记的白蛋白（FITC-白蛋白）的延长释放。通过将明胶掺入制剂中，释放持续时间显著延长：FITC-白蛋白和IL-2在超过三周的过程中释放。IL-2在整个时间内保持> 70%的活性。明胶还加速了水凝胶的凝胶化时间，并降低了它们在磷酸盐缓冲盐水中的溶胀。复合水凝胶（葡聚糖-羧甲基纤维素-明胶）在体外表现出最小的细胞毒性，并在大鼠皮下注射后表现出良性组织反应。
There is a clear need for methods of providing safe controlled release of therapeutic proteins, either to achieve and maintain high local protein concentrations, or for sustained systemic delivery. Here we have developed a protein delivery system combining in situ cross-linkable polysaccharide hydrogels with gelatin. This formulation is injectable, easy to apply, and obviates the need for organic solvents or potentially toxic cross-linking agents in the formulation process. The cross-linked polysaccharides themselves (comprising hyaluronic acid, dextran, and/or carboxymethylcellulose) provided prolonged release of fluorescently-labeled albumin (FITC-albumin). The duration of release was markedly extended by the incorporation of gelatin into the formulation: FITC-albumin and IL-2 were released over the course of more than three weeks. The IL-2 maintained > 70% activity throughout that time. Gelatin also accelerated the gelation time of the hydrogels, and reduced their swelling in phosphate buffered saline. The composite hydrogel (dextran-carboxymethylcellulose-gelatin) showed minimal cytotoxicity in vitro, and benign tissue reaction after subcutaneous injection in rats.
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DOI：
10.1016/0731-7085(92)80079-3
发表时间：
1992-08-01
影响因子：
3.4
作者：
HAMMERLING, U;HENNINGSSON, AC;SJODIN, L
通讯作者：
SJODIN, L
影响因子：
4.9
作者：
Kohane, DS;Tse, JY;Langer, R
通讯作者：
Langer, R
影响因子：
10.8
作者：
Yea, Yoon;Adil, Maroof;Kohane, Daniel S.
通讯作者：
Kohane, Daniel S.
影响因子：
5.8
作者：
Egilmez, NK;Jong, YS;Bankert, RB
通讯作者：
Bankert, RB
影响因子：
9.7
作者：
Patel, Zarana S.;Yamamoto, Masaya;Mikos, Antonios G.
通讯作者：
Mikos, Antonios G.