P2X receptors are expressed on neurons containing luteinizing hormone-releasing hormone in the mouse hypothalamus
P2X receptors are expressed on neurons containing luteinizing hormone-releasing hormone in the mouse hypothalamus
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DOI：
10.1016/j.neulet.2009.04.017
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发表时间：
2009-07
影响因子：
2.5
通讯作者：
Jiqiang Fu;Qiang Yu;W. Guo;Cheng He;G. Burnstock;Z. Xiang
中科院分区：
文献类型：
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作者：
Jiqiang Fu;Qiang Yu;W. Guo;Cheng He;G. Burnstock;Z. Xiang
采用双标记荧光免疫组织化学方法研究了小鼠下丘脑含有促黄体生成素释放激素（LHRH）的神经元上，atp门控离子通道家族成员P2X1、P2X2、P2X3、P2X4、p2x5和p2x6受体的表达。本研究表明，P2X受体亚单位的不同组合在lhrh -生成神经元的核周和轴突末端表达。首次发现P2X2、P2X4、p2x5和p2x6受体亚基在lhrh -生成神经元核周表达，P2X2和p2x6受体亚基在lhrh -生成神经元轴突末端表达。这些结果表明，ATP通过突触前和突触后不同的同质或异质P2X受体激活P2X受体可能参与前脑水平LHRH分泌的调节。
Expression of P2X1, P2X2, P2X3, P2X4, P2X5and P2X6receptors, members of a family of ATP-gated cation channels, on neurons containing luteinizing hormone-releasing hormone (LHRH) in the mouse hypothalamus was studied with double-labeling fluorescence immunohistochemistry. This study demonstrated that different combinations of P2X receptor subunits were expressed on the perikarya and axon terminals of LHRH-producing neurons. It was shown for the first time that P2X2, P2X4, P2X5and P2X6receptor subunits were expressed on the perikarya of LHRH-producing neurons and P2X2and P2X6on their axon terminals. These results suggest that activation of P2X receptors by ATP via different homomeric or heteromeric P2X receptors at both presynaptic and postsynaptic sites could be involved in the regulation of LHRH secretion at the forebrain level.