Apoptosis in human chorionic villi and decidua in normal and ectopic pregnancy
Apoptosis in human chorionic villi and decidua in normal and ectopic pregnancy
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DOI：
10.1093/molehr/4.1.87
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发表时间：
1998-01-01
影响因子：
4
通讯作者：
Nakano, R
中科院分区：
文献类型：
--
作者：
Kokawa, K;Shikone, T;Nakano, R
为了研究胚胎植入对细胞凋亡的可能影响，我们检测了人子宫内和异位妊娠时绒毛膜绒毛和蜕膜DNA的切割情况。在正常妊娠的绒毛膜绒毛和蜕膜中发现了非常有限但可检测的DNA切割。在输卵管妊娠的绒毛中出现了一种阶梯状结构，这是DNA凋亡分解的特征。输卵管妊娠蜕膜以高分子量DNA为主。低分子量DNA片段的定量分析显示，与正常妊娠相比，输卵管妊娠的绒毛组织显著增加，蜕膜组织显著减少。原位分析显示输卵管妊娠时合胞滋养细胞中凋亡细胞居多。在正常妊娠的蜕膜组织中偶见标记细胞，输卵管妊娠时标记细胞数量减少。目前的研究表明，与正常妊娠不同，输卵管妊娠的绒毛发生细胞凋亡，而蜕膜不发生细胞凋亡。我们的结果提示，植入部位可能影响人类妊娠早期细胞凋亡变化的发生。
To investigate possible effects of implantation on apoptosis, we examined the cleavage of DNA in human chorionic villi and decidua in intrauterine and ectopic pregnancy. Very limited but detectable cleavage of DNA was recognized in the chorionic villi and decidua in normal pregnancy. A ladder pattern, characteristic of the apoptotic breakdown of DNA, was present in the villi in tubal pregnancy. High molecular weight DNA was predominant in the decidua in tubal pregnancy. Quantitative analysis of low molecular weight fragments of DNA revealed a significant increase in the villous tissue, together with a significant decrease in the decidual tissue, in tubal pregnancy as compared to those in normal pregnancy. An analysis in situ revealed that apoptotic cells were predominant in the syncytiotrophoblast in tubal pregnancy. In decidual tissue, labelled cells were occasionally seen in normal pregnancy, and their numbers decreased in tubal pregnancy. The present study demonstrates that apoptosis occurs in the villi, but not in the decidua in tubal pregnancy, unlike the situation in normal pregnancy. Our results suggest that the implantation site might affect the occurrence of apoptotic changes in early pregnancy of humans.