Altered calcium homeostasis in Dahl hypertensive rats: physiological and biochemical studies.
Altered calcium homeostasis in Dahl hypertensive rats: physiological and biochemical studies.
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DOI：
10.1097/00004872-198602000-00004
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发表时间：
1986-02
影响因子：
4.9
通讯作者：
S. Umemura;D. Smyth;M. Nicar;J. Rapp;W. Pettinger
中科院分区：
文献类型：
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作者：
S. Umemura;D. Smyth;M. Nicar;J. Rapp;W. Pettinger
在原发性高血压和Okamoto-Aoki品系的自发性高血压大鼠中已经报道了异常的钙（Ca）稳态。这些异常包括尿钙排泄增加和血清离子钙（Ca 2+）降低。为了研究这些异常，我们研究了遗传上同质的高血压大鼠品系，即Dahl/Rapp盐敏感（S）和耐盐（R）大鼠（3、5、7、9、12、20和32周龄）的电解质尿排泄时间表。我们还通过测量尿cAMP排泄和腺苷酸环化酶对S和R大鼠在第2天、6周龄和28周龄时肾膜中膜受体激动剂的反应来表征肾腺苷酸环化酶-cAMP系统。第3、5和7周时，S组的尿钙排泄量高于R组（第7周时为0.48 +/- 0.04 vs 0.24 +/- 0.01 mg/mg肌酐，P <0.01）。钠和磷排泄量低于在R大鼠在5，7，9，12周，并在5，7，9，12，20和32周，分别。两组的钾排泄量相似。仅在7周龄时，S组大鼠的血浆离子钙低于R组大鼠（3.9 +/- 0.1 vs 4.5 +/- 0.1 mg/dl，P <0.01）。血浆甲状旁腺激素（PTH）在S和R大鼠之间没有差异。环AMP排泄和肾脏腺苷酸环化酶对PTH的反应，当参考基础活动时，在所有年龄的S大鼠均低于R大鼠。（250字处删节）
Abnormal calcium (Ca) homeostasis has been reported in essential hypertension and in the Okamoto-Aoki strain of spontaneously hypertensive rats. These abnormalities include increased urinary excretion of calcium and decreased ionized serum calcium (Ca2+). To pursue these abnormalities we studied the chronology of urinary excretion of electrolytes in a genetically homogeneous strain of hypertensive rat, the Dahl/Rapp salt sensitive (S) and resistant (R) rat (at ages 3, 5, 7, 9, 12, 20 and 32 weeks). We also characterized the renal adenylate cyclase-cAMP system by measuring urinary cAMP excretion and adenylate cyclase response to membrane receptor agonists in renal membranes from S and R rats at day 2 and at 6 and 28 weeks of age. Urinary calcium excretion was higher in S than in R at 3, 5 and 7 weeks (0.48 +/- 0.04 versus 0.24 +/- 0.01 mg/mg creatinine at 7 weeks, P less than 0.01). Sodium and phosphorous excretion were lower in S than in R rats at 5, 7, 9, and 12 weeks, and at 5, 7, 9, 12, 20 and 32 weeks, respectively. Potassium excretion was similar in the two groups. Plasma ionized calcium was lower in S than in R rats (3.9 +/- 0.1 versus 4.5 +/- 0.1 mg/dl, P less than 0.01) only at 7 weeks of age. Plasma parathyroid hormone (PTH) was not different between S and R rats. Cyclic AMP excretion and the renal adenylate cyclase response to PTH when referenced to basal activity was lower in S than in R rats at all ages.(ABSTRACT TRUNCATED AT 250 WORDS)