Extraction and quantification of phenolic compounds from Prunus armeniaca seed and their role in biotransformation of xenobiotic compounds
Extraction and quantification of phenolic compounds from Prunus armeniaca seed and their role in biotransformation of xenobiotic compounds
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DOI：
10.1007/s11814-016-0275-3
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发表时间：
2017-02-01
影响因子：
2.7
通讯作者：
Rehman, Fariha
中科院分区：
文献类型：
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作者：
Bibi, Ismat;Sultan, Aneela;Rehman, Fariha
目前的研究项目致力于从水果种子、果皮和蔬菜中分离出新的低成本和环保的酚类化合物，以减少大气污染。从不同的水果种子和农业废弃物中提取天然酚类化合物：亚美尼亚、桃、家蝇和小麦。对总酚含量进行定量分析，结果表明，亚美尼亚种子提取物中总酚含量最高，1 mL提取物中总酚含量为1933 μ g。植物化学筛选结果表明，种子中含有较高的生物碱、单宁、皂苷和类黄酮。种子对活性黄染料的生物转化率提高了69.89%，最大漆酶产量为322.45 IU/mL。在无天然氧化还原介质的情况下，第6天活性黄的生物降解率仅为23.34%。结果表明，使用青姜种子刺激剂，漆酶活性最高（894.4 IU/mL），去除率为99.5%。紫外可见、高效液相色谱和红外光谱分析证实了母染料转化为各种新产品。植物毒性研究表明，活性黄处理的玉米种子萌发率为0%，而活性黄处理的种子萌发率为83%，根伸长率为83%。因此，本研究表明天然酚类化合物可以作为高电位氧化还原介质，增强漆酶介导的活性黄色染料的脱色。
The current research project has been devoted to isolating new low cost and eco-friendly phenolic compounds from fruit seeds, peels and vegetables to reduce the atmospheric pollution. Natural phenolic compounds were extracted from different fruit seeds and agriculture waste: P. armeniaca, P. persica, P. domestica and Triticum aesativum. The total phenolic content was quantified, and the maximum value (1 mL extract having 1,933 mu g) was found in P. armeniaca seed extract. Phytochemical screening showed that P. armeniaca seeds contain higher amount of alkaloid, tannins, saponins and flavonoid. P. armeniaca seeds enhanced the biotransformation of reactive yellow dye up to 69.89% with maximum laccase (322.45 IU/mL) production. Biodegradation of reactive yellow was only 23.34% without natural redox mediator at sixth day of incubation. Use of P. armeniaca seed stimulators resulted in maximum laccase activity (894.4 IU/mL) with 99.5% rate of removal. UV-Vis, HPLC & FTIR analysis confirmed the transformation of parent dye into various new products. Phytotoxicity study indicated 0% germination index of Avena sativa seeds with reactive yellow, whereas 83% germination index having 100% seed germination while 83% root elongation with treated sample. Thus, the study revealed that the natural phenolic compounds could serve as high potential redox mediators for enhanced laccase-mediated decolorization of reactive yellow dye.