Effect of changes in nitrogen nutrition on the polyamine content of Alnus glutinosa
Effect of changes in nitrogen nutrition on the polyamine content of Alnus glutinosa
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氮素营养变化对赤杨多胺含量的影响
DOI：
10.1111/j.1365-3040.1991.tb00951.x
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发表时间：
1991
影响因子：
7.3
通讯作者：
A. Crozier
中科院分区：
文献类型：
--
作者：
G. Tonin;C. Wheeler;A. Crozier
.采用反相高效液相色谱荧光检测法测定了四川桤木根、根瘤和根压液中主要多胺的含量，并与9-芴基甲基氯甲酸酯和1-ADA-甘露醇胺进行柱前衍生。腐胺是所有组织和汁液的主要成分。它包括70%或更多的多胺池，但在根的硝酸钾喂养的植物，其中类似量的腐胺和亚精胺存在的水平比植物饲料（NH 4）2SO 4低五倍。根瘤中的多胺水平比根中的多胺水平高50%。当氮素营养由单纯依赖固氮转变为部分或全部利用（NH 4）2SO 4时，根系和根瘤中多胺含量变化不显著。然而，多胺含量的根压力汁液从植物的多胺几乎增加了四倍，当他们被喂以增加浓度的NH 4 NO3，虽然总的多胺含量仍然很低（5 mmol m−3汁液）。桤木根系多胺含量随树龄的变化而变化。特别是，亚精胺含量的根和结节是较高的10-相比，16周龄的植物。
. The principal polyamines in Alnus glutinosa roots, nodules and root pressure sap, putrescine, spermidine and spermine, were quantified by reversed-phase, high-performance liquid chromatography with fluorescence detection following precolumn derivatization with 9-fluorenylmethyl chloroformate and 1-ada-mantanamine. Putrescine was the major component of all tissues and sap. It comprised 70% or more of the polyamine pool except in roots of KNO3-fed plants, in which similar amounts of putrescine and spermidine were present at levels five-fold lower than plants fed (NH4)2SO4. Polyamine levels in nodules were 50% greater than in roots. The polyamine content of roots and nodules was not altered significantly when the nitrogen nutrition was changed from sole reliance on nitrogen fixation to partial or complete utilization of (NH4)2SO4. However, the polyamine content of root pressure sap from nodulated plants increased almost four-fold when they were fed with increasing concentrations of NH4NO3, although the total polyamine content remained low (5mmol m−3 sap). The polyamine content of the Alnus root system changed with plant age. In particular, the spermidine content of both roots and nodules was higher in 10- as compared to 16-week-old plants.