A family of proteins accumulating in ectoderm of sea urchin embryos specified by two related cDNA clones.
A family of proteins accumulating in ectoderm of sea urchin embryos specified by two related cDNA clones.
复制标题
由两个相关 cDNA 克隆指定的在海胆胚胎外胚层中积累的蛋白质家族。
DOI：
10.1016/0012-1606(82)90038-0
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发表时间：
1982
影响因子：
2.7
通讯作者：
Klein,WH
中科院分区：
文献类型：
--
作者：
Bruskin,AM;Bedard,PA;Tyner,AL;Showman,RM;Brandhorst,BP;Klein,WH
pSpec 1和pSpec 2两个cDNA克隆对应于海胆（stronglyocentrrotus purpuratus）幼体外胚层富集的转录本。这两个cDNA克隆，对应于多体rna的3 '端，具有相似但不同的限制图谱。这两个cDNA克隆选择的信使rna杂种在兔网织红细胞裂解液无细胞系统中被翻译成相同的10组酸性蛋白，分子量为14 - 17000道尔顿。这些体外产物在二维凝胶上与一组蛋白质具有相同或相似的位置，这些蛋白质的体内合成在外胚层高度富集。与pSpec 1和pSpec 2对应的1.5 kb和2.2 kb转录本在胚胎发育期间的患病率增加了至少100倍。这些外胚层蛋白质的合成速率也增加了100多倍，尽管变化的模式是不同的。pSpec 1和pSpec 2很可能对应于编码类似外胚层蛋白的小基因家族的mrna。这些蛋白及其mrna在原肠胚早期在外胚层富集。
Two cDNA clones, pSpec 1 and pSpec 2, had been selected previously as corresponding to transcripts greatly enriched in ectoderm of pluteus-stage larvae of the sea urchin,Stronglyocentrotus purpuratus. The two cDNA clones, corresponding to the 3′ ends of polysomal RNAs, have similar but distinct restriction maps. Messenger RNAs hybrid selected by these two cDNA clones were translated in a rabbit reticulocyte lysate cell-free system into the same set of 10 acidic proteins having molecular weights of 14–17,000 daltons. Thesein vitroproducts have positions identical or similar on two-dimensional gels to a group of proteins whose synthesisin vivois highly enriched in the ectoderm. The 1.5- and 2.2-kb transcripts corresponding to pSpec 1 and pSpec 2 increase in prevalence by at least 100-fold during embryonic development. The rates of synthesis of these ectoderm proteins also increase by over 100-fold, though the patterns of change are distinct. It is likely that pSpec 1 and pSpec 2 correspond to mRNAs which are part of a small family of genes coding for the group of similar ectoderm proteins. These proteins and their mRNAs are enriched in ectoderm by the early gastrula stage.