Plant Regeneration from Leaf-derived Callus Cultures of Primrose (Primula vulgaris)
Plant Regeneration from Leaf-derived Callus Cultures of Primrose (Primula vulgaris)
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DOI：
10.21273/hortsci.51.5.558
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发表时间：
2016-05-01
期刊：
影响因子：
1.9
通讯作者：
Gilmartin, Philip M.
中科院分区：
文献类型：
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作者：
Hayta, Sadiye;Smedley, Mark A.;Gilmartin, Philip M.
报春花属植物的高效微繁殖对于基础科学研究和商业应用都是重要的。普通报春（Primula vulgaris（thuds））与其他报春科植物一样，沿着具有两种不同的两性花。鉴定控制异形花发育的基因的研究需要繁殖花突变体，而有效的再生是植物转化的关键要求。包括P. vulgaris栽培品种和P. xpolyantha杂交种在内的几个品种是欧洲、美国和日本的重要园艺作物，半重瓣/重瓣报春花品种提供了高端产品。然而，无性繁殖不育双型，并作为一种手段，以增加F1种子生产的近交亲本植株的数量是缓慢的。微繁殖提供了快速有效地增加这些品种的最有效的方法。迄今为止，大多数报春花微繁殖方案需要外植体材料来自体外生长的幼苗或使用花的部分作为供体材料与季节限制。因此，建立了一种有效的、高效的、从普通菜豆的成年叶片来源的外植体通过间接器官发生进行离体再生的方法。将普通菜豆叶片外植体暴露于含有合成细胞分裂素、噻苯隆（TDZ）和生长素[1-萘乙酸（NAA）或2，4-二氯苯氧乙酸（2，4-D）]的培养基中，导致未分化细胞增殖，随后分化生长为芽器官发生。银硝酸盐提高了体外愈伤组织的生长，并进一步增加了芽再生，高达72%的外植体产生芽。再生植株在7个月内发育正常，并产生正常的可育花。该体系还成功地应用于无菌重瓣白花夏枯草‘Sue Jervis’的微繁。这里报告的协议，使繁殖的P. vulgaris没有季节性限制或破坏有价值的父母供体材料。该方案，进一步发展，有可能支持的报春花转化系统的开发，这将是在花发育和抗病性的研究在实验室种植的植物的价值。
Efficient micropropagation of Primula species is important both for fundamental scientific studies and commercial applications. Primula vulgaris (thuds), along with other Primulaceae species, exhibits floral heteromorphy with two distinct forms of hermaphroditic flower. Studies to identify genes that control heteromorphic flower development require propagation of floral mutants, and efficient regeneration is a key requirement for plant transformation. Several species, including P. vulgaris cultivars and P. xpolyantha hybrids, are important horticultural crops in Europe, United States, and Japan and semidouble/double Primula varieties offer a high-end product. Vegetative propagation of sterile double forms, and as a means to increase numbers of inbred parent plants for F1 seed production is, however, slow. Micropropagation offers the most efficient way of increasing these varieties quickly and efficiently. To date, most Primula micropropagation protocols require explant material derived from in vitro grown seedlings or use floral parts as donor material with seasonal limitations. Therefore, an effective and efficient protocol was developed for in vitro regeneration of P. vulgaris via indirect organogenesis from adult leaf derived explants. Exposure of leaf explants of P. vulgaris to media containing synthetic cytokinin, thidiazuron (TDZ), and auxin [1-naphthylacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D)] resulted in undifferentiated cell proliferation followed by differentiated growth as shoot organogenesis. Silver nitrate improved in vitro callus growth and increased shoot regeneration further, with up to 72% of explants producing shoots. Regenerated plants developed normally and produced normal fertile flowers within 7 months. The system was also successfully applied for the micropropagation of sterile double-flowered P. vulgaris 'Sue Jervis'. The protocol reported here enables propagation of P. vulgaris without seasonal limitation or destruction of valuable parent donor material. The protocol, with further development, has the potential to underpin development of a transformation system for Primula, which would be of value in studies on flower development and disease resistance in laboratory grown plants.