Class II MHC antigens in the rat digestive system. Normal distribution and induced expression after interferon-gamma treatment in vivo.
Class II MHC antigens in the rat digestive system. Normal distribution and induced expression after interferon-gamma treatment in vivo.
复制标题
大鼠消化系统中的 II 类 MHC 抗原。
作者：
B. Steiniger;P. Falk;M. Lohmüller;P. H. van der Meide
用免疫组织化学方法研究了LEW大鼠消化系统中正常和干扰素-γ诱导的II类MHC抗原的表达。在正常状态下，II类分子存在于间质树突状细胞、B淋巴细胞和上皮细胞中。上皮II类表达仅限于小肠某些部分的肠上皮细胞和唾液腺中的一些导管上皮细胞。连续静脉输注干扰素-γ（IFN-γ）3天后，诱导II类MHC抗原在大血管内皮细胞和表面上皮细胞的舌头，食道和前心室。在胃腺II类分子出现在粘液颈细胞和壁细胞，而相邻的粘液表面细胞和主细胞没有获得II类反应。所有的肠上皮细胞，包括以前阴性的结肠上皮，诱导表达II类抗原。在唾液腺中，II类抗原出现在所有导管上皮细胞中。在腮腺中诱导浆液性腺泡细胞，但在颌下腺和胰腺中浆液性腺上皮保持阴性。因此，我们的研究表明，IFN-γ在体内对II类MHC抗原表达的影响取决于单个细胞的分化途径。在大鼠中的正态分布表明，II类MHC抗原可能发挥作用，在肽转运通过专门的上皮细胞。IFN治疗后这种功能是否增强仍有待确定。
The normal and interferon-gamma induced expression of class II MHC antigens was investigated immunohistologically in the digestive system of LEW rats. In the normal state class II molecules were present in interstitial dendritic cells, B lymphocytes and epithelial cells. Epithelial class II expression was restricted to enterocytes in certain portions of the small intestine and to some duct epithelia in salivary glands. After continuous intravenous infusion of interferon-gamma (IFN-gamma) for 3 days, class II MHC antigens were induced in large vessel endothelium and in the surface epithelia of the tongue, oesophagus and proventricle. In the gastric glands class II molecules appeared in mucous neck cells and in parietal cells, while adjacent mucous surface cells and chief cells did not acquire class II reactivity. All enterocytes, including the previously negative colonic epithelium, were induced to express class II antigens. In the salivary glands class II antigens appeared in all duct epithelia. Serous acinar cells were induced in the parotids, but in the submandibular glands and in the pancreas the serous gland epithelium stayed negative. Our study thus shows that the effects of IFN-gamma on class II MHC antigen expression in vivo depend on the differentiation pathway of the individual cell. The normal distribution in rats suggest that class II MHC antigens may play a role in peptide transport across specialized epithelia. It remains to be determined whether such a function is enhanced after IFN treatment.