The Regenerative Role of Gelatin in PLLA Electrospun Membranes for the Treatment of Chronic Massive Rotator Cuff Injuries
The Regenerative Role of Gelatin in PLLA Electrospun Membranes for the Treatment of Chronic Massive Rotator Cuff Injuries
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明胶在 PLLA 电纺膜治疗慢性大面积肩袖损伤中的再生作用
DOI：
10.1002/mabi.202100281
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发表时间：
2021
影响因子：
4.6
通讯作者：
Kun Wang
中科院分区：
文献类型：
--
作者：
Chang Liu;Shihai Jiang;Yu Wu;Libiao Liu;Shouwen Su;Tangzhao Liang;Ronghan He;Zeyue Guo;Yuanyuan Zhang;Zhidong Lin;Wei Niu;Lei Zhu;Tao Xu;Kun Wang
慢性大面积肩袖撕裂（cmrct）不能再生天然肌腱组织导致术后高撕裂率。明胶是一种水解形式的胶原蛋白，具有生物活性和生物相容性。本研究旨在探讨明胶与聚乳酸（PLLA）纤维膜结合促进cmrct愈合的适用性。采用静电纺丝技术制备了聚乳酸/明胶静电纺丝膜。对傅里叶变换红外、静态接触角进行了顺序测试。用大鼠肌腱成纤维细胞和人脐带内皮细胞（HUEVCs）系进行细胞相容性评价。建立cmrct大鼠模型，分为三组（假手术组、修复组和增强组）进行组织形态学和生物力学评价。通过静电纺丝技术将明胶成功地整合到聚乳酸纤维膜中。体外研究表明，PGEM对大鼠肌腱成纤维细胞和HUEVCs具有良好的细胞相容性。体内研究发现，在cmrct大鼠模型中，PGEM的应用显著促进了I型胶原纤维的形成，并增强了修复肌腱的生物力学性能。综上所述，明胶可促进肌腱成纤维细胞和HUEVCs在PLLA纤维膜上的粘附、迁移和增殖，PGEM具有广阔的临床应用前景。
Failing to regenerate native tendon tissue in chronic massive rotator cuff tears (CMRCTs) results in high retear rates after surgery. Gelatin is a hydrolyzed form of collagen which is bioactive and biocompatible. This study intends to investigate the suitability of integrating gelatin to poly (l‐lactic acid) (PLLA) fibrous membranes for promoting the healing of CMRCTs. PLLA/Gelatin electrospun membranes (PGEM) are fabricated using electrospinning technology. The fourier transform infrared, static contact angles are tested sequentially. Cytocompatibility is evaluated with rat tendon fibroblasts and human umbilical endothelial cells (HUEVCs) lines. CMRCTs rat models are established and assigned into three groups (the sham group, the repaired group, and the augmentation group) to perform histomorphological and biomechanical evaluations. Gelatin is successfully integrated into PLLA fibrous membranes by the electrospinning technique. In vitro studies indicate that PGEM shows a great cytocompatibility for rat tendon fibroblasts and HUEVCs. In vivo studies find that applications of PGEM significantly promote well‐aligned collagen I fibers formation and enhance biomechanical properties of the repaired tendon in CMRCTs rat models. In summary, gelatin promotes tendon fibroblasts and HUEVCs adhesion, migration, and proliferation on the PLLA fibrous membranes, and PGEM may provide a great prospect for clinical application.