Anti-inflammatory and related pharmacological activities of cultured mycelia and fruiting bodies of Cordyceps militaris
Anti-inflammatory and related pharmacological activities of cultured mycelia and fruiting bodies of Cordyceps militaris
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DOI：
10.1016/j.jep.2004.10.009
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发表时间：
2005-01-15
影响因子：
5.4
通讯作者：
Park, EH
中科院分区：
文献类型：
--
作者：
Won, SY;Park, EH
本研究旨在阐明北虫夏草的药理活性。制备了北虫夏草培养菌丝体和子实体的70%乙醇提取物。CME能直接清除稳定的自由基二苯基-2-苦基肼(DPPH)，表明其具有抗氧化活性。CME和FBE对巴豆油诱导的小鼠耳肿胀均有局部抗炎活性。用角叉菜胶致小鼠水肿法评价其急性抗炎活性，在扭体实验中也发现其具有较强的抗伤害性。CME和FBE对鸡胚绒毛尿囊膜(CAM)血管生成有明显的抑制作用，且呈剂量依赖关系。北虫夏草的代谢物虫草素似乎至少是其抗炎和抗血管生成活性的部分原因。在小鼠巨噬细胞系RAW 264.7中，CME浓度依赖地抑制脂多糖刺激下NO的产生和iNOS的表达。简而言之，我们证明了北虫夏草具有抗炎和抗伤害性活性，以及相关的抗氧化、抗血管生成和NO生成抑制活性。(C)2004年，爱思唯尔爱尔兰有限公司出版。
This study aimed to elucidate pharmacological activities of Cordyceps militaris. The 70% ethanolic extracts of cultured mycelia (CME) and fruiting bodies (FBE) of Cordyceps militaris were prepared. CME was able to directly scavenge the stable free radical diphenyt-2-picrylhydrazyl (DPPH), indicating its antioxidant activity. Both CME and FBE showed topical anti-inflammatory activity in the croton oil-induced ear edema in mice. CME was found to contain acute anti-inflammatory activity, which was evaluated using the carrageenin-induced edema, and also strong antinociceptive activity in writhing test. CME and FBE contain potent inhibitory activity on the chick embryo chorioallantoic membrane (CAM) angiogenesis in a dose-dependent manner. Cordycepin, a metabolite of Cordyceps militaris, appeared to be at least partly responsible for its anti-inflammatory and anti-angiogenic activities. CME concentration-dependently inhibited the NO production and iNOS expression upon stimulation by lipoposaccharide in RAW 264.7, a murine macrophage cell line. In brief, we demontrate that Cordyceps militaris possesses anti-inflammatory and antinociceptive activites, and related antioxidant, anti-angiogenic, and NO production-inhibitory activities. (C) 2004 Published by Elsevier Ireland Ltd.