Effect of feeding behavior on circadian regulation of endothelin expression in mouse colon
Effect of feeding behavior on circadian regulation of endothelin expression in mouse colon
复制标题
DOI：
10.1016/j.lfs.2014.06.022
复制
发表时间：
2014-11-24
期刊：
影响因子：
6.1
通讯作者：
Saida, Kaname
中科院分区：
文献类型：
--
作者：
Kozakai, Takaharu;Sakate, Mitsue;Saida, Kaname
目的：内皮素（ET）系统在肠道中的功能、调控和基因表达尚不清楚。本研究采用小鼠自由进食、禁食48 h和禁食后再进食的方法，研究了小鼠结肠ET-1基因表达与进食时间和生物钟的关系。禁食增加了结肠上皮组织ET-1基因表达的水平，同时保持了其节律性。然而，再喂养降低了ET-1基因的表达，抑制了节律振荡，并且昼夜节律钟周期1和周期2（Per 1和Per 2）的基因表达的节律性也发生了变化。此外，再喂养引起的ET-1基因表达的下降被六烃季铵和阿托品预处理所阻断。结肠中ET-1基因表达的每日变化依赖于通过自主神经系统的进食时间表，在自由进食和禁食但不重新进食的条件下与外周昼夜振荡器同步。ET-1基因在近端结肠表达的减少是通过神经系统介导的，意义：ET-1具有重要的生理作用，依赖于进食行为。(C)2014作者爱思唯尔公司出版这是CC BY-NC-ND许可下的开放获取文章（http：//creativecommons.org/licenses/by-nc-nd/3.0/）。
Aims: The function, regulation and gene expression of the endothelin (ET) system in the intestine is not well understood. We investigated the dependence on feeding schedule and biological clock of the regulation of ET-1 gene expression in mouse colon.Main methods: Mice were fed freely, fasted for 48 h and re-fed after fasting.Key findings: Where indicated ET-1 gene expression was highest in the colon compared with other tissues examined in fasted mice. Fasting increased the level, while maintaining the rhythmicity, of ET-1 gene expression in epithelial colonic tissue. Re-feeding, however, decreased ET-1 gene expression and suppressed rhythmic oscillation, and the rhythmicity also changed for gene expression for circadian clocks, period-1 and period-2 (Per1 and Per2). Furthermore, the decrease in ET-1 gene expression induced by re-feeding was blocked by pre-treatment with hexamethonium and atropine. The daily change in ET-1 gene expression in colon, which depends on feeding schedule via the autonomic nervous system, is synchronized with peripheral circadian oscillators under conditions of free feeding and fasting but not re-feeding. The decrease in ET-1 gene expression in the proximal colon induced by re-feeding occurs via the nervous system.Significance: ET-1 plays an important physiological role, which is dependent on feeding behavior. (C) 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).