Comparative transcriptome analysis of Desulfovibrio vulgaris grown in planktonic culture and mature biofilm on a steel surface
Comparative transcriptome analysis of Desulfovibrio vulgaris grown in planktonic culture and mature biofilm on a steel surface
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DOI：
10.1007/s00253-007-1014-9
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发表时间：
2007-06
影响因子：
5
通讯作者：
Weiwen Zhang;D. Culley;L. Nie;J. Scholten
中科院分区：
文献类型：
--
作者：
Weiwen Zhang;D. Culley;L. Nie;J. Scholten
硫酸盐还原菌（SRB）在金属表面的生物膜积累可能导致铁的严重腐蚀。为了在分子水平上了解这一过程，本研究采用全基因组寡核苷酸芯片技术检测了钢铁表面脱硫弧菌（Desulfovibrio vulgaris）的增殖种群和成熟生物膜之间的差异表达模式。统计分析显示，472个基因的差异表达（1.5倍或更多，aq值小于0.025），通过比较生物膜细胞和嗜酸性细胞。在差异表达的基因中有几个对应于在许多好氧细菌生物膜中鉴定的基因（即，假单胞菌属和大肠杆菌），如编码鞭毛蛋白的基因，鞭毛马达开关蛋白，参与细胞运动的趋化蛋白，以及参与胞外多糖生物合成的基因。此外，D. vulgaris表现出参与蛋白质合成、能量代谢和硫酸盐还原的基因以及参与一般应激反应的基因的转录降低。这些发现都与早期的建议一致，即生物膜细胞的平均生理学与生长减少的细胞相似。最值得注意的是，在D. vulgaris生物膜。虽然它们的功能仍然是未知的，这些基因在生物膜中的较高表达可能涉及在钢表面上的多细胞聚生体的形成和维持中的重要作用。该研究提供了与aD形成和维持相关的代谢网络的见解。vulgaris生物膜on a steel钢surface表面.
Biofilm build-up of sulphate-reducing bacteria (SRB) on metal surfaces may lead to severe corrosion of iron. To understand the processes at molecular level, in this study, a whole-genome oligonucleotide microarray was used to examine differential expression patterns between planktonic populations and mature biofilm ofDesulfovibrio vulgarison a steel surface. Statistical analysis revealed that 472 genes were differentially expressed (1.5-fold or more with aqvalue less than 0.025) by comparing the biofilm cells with the planktonic cells. Among the differentially expressed genes were several that corresponded to genes identified in many aerobic bacterial biofilms (i.e.,Pseudomonasspecies andEscherichia coli) such as genes encoding flagellin, a flagellar motor switch protein, chemotaxis proteins involved in cell motility, as well as genes involved in exopolysaccharide biosynthesis. In addition, the biofilm-bound cells ofD. vulgarisexhibited decreased transcription of genes involved in protein synthesis, energy metabolism and sulfate reduction, as well as genes involved in general stress responses. These findings were all consistent with early suggestion that the average physiology of the biofilm cells were similar to cells reduced in growth. Most notably, up-regulation of large number of outer membrane proteins was observed in theD. vulgarisbiofilm. Although their function is still unknown, the higher expression of these genes in the biofilm could implicate important roles in the formation and maintenance of multi-cellular consortium on a steel surface. The study provided insights into the metabolic networks associated with the formation and maintenance of aD. vulgarisbiofilm on a steel surface.