DNA duplication is essential for the repair of gastrointestinal perforation in the insect midgut.
DNA duplication is essential for the repair of gastrointestinal perforation in the insect midgut.
复制标题
DNA复制对于昆虫中肠胃肠穿孔的修复至关重要
DOI：
10.1038/srep19142
复制
发表时间：
2016-01-12
影响因子：
4.6
通讯作者：
Ling E
中科院分区：
文献类型：
--
作者：
Huang W;Zhang J;Yang B;Beerntsen BT;Song H;Ling E
无脊椎动物具有通过不同机制修复皮肤和肠道伤口的能力。胃肠道穿孔是人体胃肠道系统上的一个洞，是一种严重的疾病，需要手术修复穿孔以防止腹部脓肿或败血症。在这里，我们报告的修复胃肠道穿孔所造成的针刺伤口在家蚕幼虫中肠。在昆虫肠穿孔后，仅观察到弱的免疫应答，因为在针刺幼虫中肠后6h收集的血浆部分抑制了大肠杆菌的生长。然而，循环血细胞确实聚集在针刺伤口上形成结痂。虽然在伤口部位没有观察到细胞分裂和凋亡，但针刺显著增强了伤口周围细胞中的DNA复制，这对修复中肠穿孔至关重要。由于针刺中肠引起的修复能力和有限的免疫应答，该方法成功地用于将小化合物（在本研究中为乙醇）注射到昆虫中肠中。因此，这种昆虫肠道的针刺创伤可以被开发用于筛选将来用作肠道化疗剂的化合物。
Invertebrate animals have the capacity of repairing wounds in the skin and gut via different mechanisms. Gastrointestinal perforation, a hole in the human gastrointestinal system, is a serious condition and surgery is necessary to repair the perforation to prevent an abdominal abscess or sepsis. Here we report the repair of gastrointestinal perforation made by a needle-puncture wound in the silkworm larval midgut. Following insect gut perforation, only a weak immune response was observed because the growth ofEscherichia colialone was partially inhibited by plasma collected at 6 h after needle puncture of the larval midgut. However, circulating hemocytes did aggregate over the needle-puncture wound to form a scab. While, cell division and apoptosis were not observed at the wound site, the needle puncture significantly enhanced DNA duplication in cells surrounding the wound, which was essential to repair the midgut perforation. Due to the repair capacity and limited immune response caused by needle puncture to the midgut, this approach was successfully used for the injection of small compounds (ethanol in this study) into the insect midgut. Consequently, this needle-puncture wounding of the insect gut can be developed for screening compounds for use as gut chemotherapeutics in the future.