SELECTIVE ADHESION OF EMBRYONAL CARCINOMA-CELLS AND DIFFERENTIATED CELLS BY CA2+-DEPENDENT SITES
SELECTIVE ADHESION OF EMBRYONAL CARCINOMA-CELLS AND DIFFERENTIATED CELLS BY CA2+-DEPENDENT SITES
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DOI：
10.1016/0012-1606(81)90157-3
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发表时间：
1981-01-01
影响因子：
2.7
通讯作者：
OKADA, TS
中科院分区：
文献类型：
--
作者：
TAKEICHI, M;ATSUMI, T;OKADA, TS
[小鼠]胚胎癌细胞和成纤维细胞之间的粘附的特异性进行了研究。胚胎癌细胞具有需要Ca 2+的细胞间粘附位点（CDS）。这些位点对蛋白酶敏感，但在Ca 2+存在下对它们具有抗性。具有类似蛋白酶敏感性的CDS存在于成纤维细胞中。当不同品系的胚胎癌细胞混合时，它们通过CDS非选择性地彼此粘附。CDS的非选择性粘附发生在不同细胞系的成纤维细胞之间。当胚胎癌和成纤维细胞混合时，它们优先粘附于同型细胞。针对F9细胞（胚胎癌的nullipotent系）产生的抗体的Fab片段抑制胚胎癌细胞之间的粘附，但不抑制成纤维细胞之间的粘附。Fab的这种抑制活性被具有CDS的胚胎癌细胞吸收，但不被具有CDS的成纤维细胞或通过实验去除CDS的胚胎癌细胞吸收。放射性碘标记的细胞表面蛋白的SDS-聚丙烯酰胺凝胶电泳显示，在胚胎癌细胞中，140 K-道尔顿组分的存在与CDS的存在相关，而在成纤维细胞中，150 K-道尔顿组分的存在与CDS的存在相关。这些结果表明，CDS在胚胎癌和成纤维细胞包括不同的分子。
The specificity of adhesion between [mouse] embryonal carcinoma cells and fibroblastic cells of various origins was studied. Embryonal carcinoma cells have intercellular adhesion sites requiring Ca2+ (CDS). These sites were sensitive to proteases but resistant to them in the presence of Ca2+. CDS with a similar protease sensitivity is present in fibroblastic cells. When embryonal carcinoma cells of different lines were mixed, they adhered to each other nonselectively by CDS. Nonselective adhesion by CDS occurred between fibroblastic cells of various lines. When embryonal carcinoma and fibroblastic cells were mixed, they preferentially adhered to homotypic cells. Fab fragments of antibodies raised against F9 cells (a nullipotent line of embryonal carcinoma) inhibited the adhesion between embryonal carcinoma cells but not between fibroblastic cells. This inhibitory activity of Fab was absorbed with embryonal carcinoma cells with CDS but not with fibroblastic cells with CDS or embryonal carcinoma cells from which CDS was experimentally removed. SDS-polyacrylamide gel electrophoresis of radioiodinated cell surface proteins showed that the presence of a 140K[kilo]-dalton component correlated with the presence of CDS in embryonal carcinoma cells, while the presence of a 150K-dalton component correlated with the presence of CDS in fibroblastic cells. These results suggest that CDS in embryonal carcinoma and fibroblastic cells comprise distinct molecules.