Proteomic analysis of somatic embryogenesis in Valencia sweet orange (Citrus sinensis Osbeck)
Proteomic analysis of somatic embryogenesis in Valencia sweet orange (Citrus sinensis Osbeck)
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巴伦西亚甜橙（Citrus sinensis Osbeck）体细胞胚胎发生的蛋白质组学分析
DOI：
10.1007/s00299-008-0633-7
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发表时间：
2009-02-01
影响因子：
6.2
通讯作者：
Deng, Xiuxin
中科院分区：
文献类型：
--
作者：
Pan, Zhiyong;Guan, Rui;Deng, Xiuxin
采用双向凝胶电泳法结合基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF-MS)对瓦伦西亚甜橙的体细胞胚胎发生进行了研究。在柑橘SE的5个时间点(胚胎起始后0、1、2、3、4周)，共鉴定出24个差异表达蛋白，涵盖球状、心形/鱼雷和子叶形胚期。这些蛋白的一般表达模式与柑橘SE的4周时的表达模式一致。本研究最显著的特点是预测了参与谷胱甘肽(GSH)代谢和抗氧化应激的五种蛋白质，它们在SE过程中表现出不同的表达模式。基于氧化胁迫已被证实可以增强SE，抗氧化蛋白的优先表达表明它们可能在柑橘SE中具有发育作用。还鉴定了一些参与细胞分裂、光合作用和解毒作用的蛋白质，并讨论了它们在柑橘SE中的可能作用。
Two dimensional gel electrophoresis combined with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was employed to study the somatic embryogenesis (SE) in Valencia sweet orange (Citrus sinensis Osbeck). Twenty-four differentially expressed proteins were identified at five time points of citrus SE (0, 1, 2, 3, 4 weeks after embryo initiation) covering globular, heart/torpedo and cotyledon-shaped embryo stages. The general expression patterns for these proteins were consistent with those appeared at 4 weeks of citrus SE. The most striking feature of our study was that five proteins were predicted to be involved in glutathione (GSH) metabolism and anti-oxidative stress, and they exhibited different expression patterns during SE. Based on that oxidative stress has been validated to enhance SE, the preferential representation for anti-oxidative proteins suggests that they could have a developmental role in citrus SE. Some proteins involved in cell division, photosynthesis and detoxification were also identified, and their possible roles in citrus SE were discussed.