Detection of vertebrate-type sterid hormones and their converting activity in the neogastropid, Thais clavigera
Detection of vertebrate-type sterid hormones and their converting activity in the neogastropid, Thais clavigera
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脊椎动物新腹足类甾体激素及其转化活性的检测
DOI：
10.1093/mollus/eys001
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Miura T.
中科院分区：
文献类型：
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作者：
Goto;Y.;Kajiwara;M.;Yanagisawa;Y.;Hirose;H.;Yoshimi;T.;Umemura;M.;Nakano;H.;Takahashi;S.;Shida;Y.;Iguchi;T.;Takahashi;Y.;Miura T.
对软体动物生殖系统的调节的知识是有限的。许多研究已经描述了雌激素是如何调节软体动物性腺的发育和成熟的。在软体动物中检测到脊椎动物型雌激素，17β-雌二醇，其在性腺中的浓度随着生殖周期而变化。然而，目前还不清楚，如果软体动物的性腺组织中芳香化酶活性相对较高。Thais clavigera已被证明具有优先表达的雌激素受体基因在卵巢和神经节，这表明与性腺成熟和雌激素受体基因的表达。因此，探讨棒毛锥虫体内是否存在脊椎动物型性类固醇激素和芳香化酶活性是一个有趣的研究课题。我们应用气相色谱-质谱联用技术检测了黑腹果蝇性腺组织中的类固醇激素。clavigera和鉴定了睾酮、雄烯二酮、17β-雌二醇和雌酮。雄烯二酮和睾酮转化为雌酮和17β-雌二醇的芳香化酶活性在性腺中明显高于消化腺。这些数据提供了新腹足类动物内分泌系统的见解，促进了芳香化酶基因的分子证据。
Knowledge of the regulation of molluscan reproductive systems is limited. Many studies have described how the development and maturation of molluscan gonads are regulated by oestrogen. Vertebrate-type oestrogen, 17β-oestradiol, has been detected in molluscs and its concentration in the gonads has been suggested to change in conjunction with reproductive cycles. However, it is unclear if molluscan gonadal tissues are relatively high in aromatase activities.Thais clavigerahas been shown to possess a preferential expression of the oestrogen receptor gene in the ovary and ganglia, suggesting an association with gonadal maturation and expression of the oestrogen receptor gene. Therefore, it is interesting to explore the existence of vertebrate-type sex steroid hormones and aromatase activity inT. clavigera. We applied gas chromatography–mass spectrometry techniques to detect steroids in the gonadal tissues ofT. clavigeraand identified testosterone, androstenedione, 17β-oestradiol and oestrone. The aromatase activities converting androstenedione and testosterone into oestrone and 17β-oestradiol were much higher in gonads than in digestive glands. These data provide insights into the endocrinological system of neogastropods, prompting molecular evidence for the aromatase gene.