Antimicrobial activity and mechanism of Larch bark procyanidins against Staphylococcus aureus
Antimicrobial activity and mechanism of Larch bark procyanidins against Staphylococcus aureus
复制标题
落叶松树皮原花青素对金黄色葡萄球菌的抗菌活性及机制
DOI：
10.1093/abbs/gmx112
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发表时间：
2017-12-01
影响因子：
3.7
通讯作者：
Jia, Yan
中科院分区：
文献类型：
--
作者：
Li, Xinchao;He, Congfen;Jia, Yan
落叶松树皮原花青素（LBPCs）不仅具有抗氧化和抗肿瘤作用，而且具有很强的抑菌作用。然而，LBPC的抗菌机制尚不清楚。本文从形态结构、细胞壁和细胞膜、必需蛋白、遗传物质等方面研究了LBPC对金黄色葡萄球菌的抑菌作用及其机制。结果表明，LBPC对细菌生长有明显的抑制作用，其最低抑菌浓度为1.75mg/ml。LBPC处理显著改变了细菌的形态，细胞壁和细胞膜被破坏。胞外碱性磷酸酶含量、菌液电导率、膜系统Na+/K+-ATP酶和Ca ~（2+）-ATP酶活性均增加。在能量代谢系统中，琥珀酸脱氢酶、苹果酸脱氢酶和三磷酸腺苷酶（ATP酶）的活性均降低，导致代谢减慢，细菌生长受到抑制。细菌蛋白质含量和组成的变化表明蛋白质表达系统受到影响。此外，发现LBPC与DNA凹槽结合形成复合物。因此，LBPC对S.金黄色葡萄球菌，并能杀死S.金黄色葡萄球菌通过破坏细胞壁和细胞膜的完整性和渗透性，影响蛋白质合成，并结合DNA。
Larch bark procyanidins (LBPCs) have not only antioxidant and antitumor properties, but also strong bacteriostatic effects. However, it is not clear about the antibacterial mechanisms of LBPC. In this work, the antibacterial effects and mechanisms of LBPC on Staphylococcus aureus were studied in the aspects of morphological structure, cell wall and membrane, essential proteins, and genetic material. The results showed that LBPC effectively inhibited bacterial growth at a minimum inhibitory concentration of 1.75 mg/ml. Bacterial morphology was significantly altered by LBPC treatment, with the cell walls and membranes being destroyed. Extracellular alkaline phosphatase content, bacterial fluid conductivity, and Na+/K+-ATPase and Ca2+-ATPase activities in the membrane system were all increased. In the energy metabolic systems, the activities of succinate dehydrogenase, malate dehydrogenase, and adenosine triphosphatase (ATPase) were all decreased, resulting in a slowdown of metabolism and bacterial growth inhibition. Changes of protein content and composition in the bacteria suggested that the protein expression system was affected. In addition, LBPC was found to bind to DNA grooves to form complexes. Thus, LBPC has a very strong inhibitory effect on S. aureus and can kill S. aureus by destroying the integrity and permeability of the cell wall and cell membrane, affecting protein synthesis, and binding to DNA.