Flow control effect of necrostatin-1 on cell death of the NRK-52E renal tubular epithelial cell line.
Flow control effect of necrostatin-1 on cell death of the NRK-52E renal tubular epithelial cell line.
复制标题
necrostatin-1对NRK-52E肾小管上皮细胞系细胞死亡的流量控制作用
DOI：
10.3892/mmr.2017.6556
复制
发表时间：
2017-07
影响因子：
3.4
通讯作者：
Shi W
中科院分区：
文献类型：
--
作者：
Luo J;Tao Y;Liang X;Chen Y;Zhang L;Jiang F;Liu S;Ye Z;Li Z;Shi W
急性肾损伤（阿基）时肾小管上皮细胞（RTEC）凋亡和坏死性凋亡发生，并可通过多种方式进行调控。本研究确定了Necrostatin-1（Nec-1）对RTEC的保护作用，通过流量控制样作用。结果建立了大鼠NRK-52 E RTECs缺氧缺血损伤模型，该模型使用肿瘤坏死因子-α，然后用抗霉素A和泛半胱天冬酶通路阻断剂苄氧羰基-Val-Ala-Asp-氟甲基酮消耗ATP。用Nec-1预处理细胞后，观察到细胞器膨胀、碎片抑制和细胞活力改善，同时微管相关蛋白1A/1B-轻链3-II表达减少。Nec-1参与细胞损伤和死亡过程中的流动控制。总之，本研究表明，Nec-1提供了保护作用，并在NRK-52 E细胞模型中预防阿基中发挥重要作用。需要进一步的研究来充分研究Nec-1在体内阿基发展中的作用。
Apoptosis and necroptosis occur in renal tubular epithelial cell (RTEC) death in acute kidney injury (AKI), and may be regulated by several methods. The present study identified a protective effect of necrostatin-1 (Nec-1) on RTECs via a flow-control-like effect. The results established a hypoxic-ischemic injury model of rat NRK-52E RTECs using tumour necrosis factor-α followed by ATP depletion with antimycin A and the pan-caspase pathway blocker, benzyloxycarbonyl-Val-Ala-Asp-fluoro-methylketone. Following pre-treatment of cells with Nec-1, cell organelle inflation, fragmentation inhibition and improved cell viability were observed with a parallel reduced expression of microtubule-associated protein 1A/1B-light chain 3-II. Nec-1 was involved in flow control in the process of cell injury and death. In conclusion, the present study indicated that Nec-1 provides a protective effect and serves an important role in the prevention of AKI in an NRK-52E cell model. Further studies will be required to fully investigate the role of Nec-1 in the development of AKI in vivo.