Altered composition of the cumulus-oocyte complex matrix during in vitro maturation of oocytes
Altered composition of the cumulus-oocyte complex matrix during in vitro maturation of oocytes
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DOI：
10.1093/humrep/dem277
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发表时间：
2007-11-01
影响因子：
6.1
通讯作者：
Russell, Darryl L.
中科院分区：
文献类型：
--
作者：
Dunning, Kylie R.;Lane, Michelle;Russell, Darryl L.
背景：哺乳动物卵母细胞体外成熟（IVM）作为促性腺激素治疗的替代方案，对接受辅助生殖的患者具有潜在的健康益处。该方法也可用于研究卵母细胞和早期胚胎的发育过程。然而，经过IVM的卵母细胞的能力远低于体内成熟的卵母细胞。优化IVM成功的努力集中在复制体内时间、激素环境和与成熟卵母细胞相关的卵丘细胞反应。我们之前已经确定了两种细胞外基质蛋白，蛋白酶Adamts1和透明质酸结合蛋白聚糖Versican，它们是由壁粒细胞选择性地结合到排卵周卵母细胞复合体（COC）中产生的。方法：在表皮生长因子和/或FSH存在下培养小鼠COC。采用实时荧光定量PCR和Western blot检测mRNA和蛋白含量，并与体内COC进行比较。结果：在表皮生长因子、卵泡刺激素或两者同时存在的情况下，体外培养6或20小时的小鼠COCs mRNA减少了10倍(P
BACKGROUND: In vitro maturation (IVM) of mammalian oocytes has potential health benefits for patients undergoing assisted reproduction as an alternative to gonadotrophin treatment. This procedure is also useful for studying the process of oocyte and early embryo development. However, oocytes undergoing IVM have much lower competence than in vivo matured oocytes. Efforts to optimize IVM success have focused on replicating in vivo timing, hormonal milieu and cumulus cell responses associated with maturing oocytes. We have previously identified two extracellular matrix proteins, the protease Adamts1 and hyaluronan-binding proteoglycan Versican, produced by mural granulosa cells that selectively incorporate into the periovulatory cumulus-oocyte complex (COC). METHODS: Murine COC were cultured in the presence of epidermal growth factor and/or FSH. mRNA and protein were measured by real time PCR and Western blot and compared to in vivo derived COC. RESULTS: COCs from mice that underwent IVM for 6 or 20 h in the presence of epidermal growth factor, FSH or in combination had a >10-fold reduction in mRNA (P