HOG-MAPK Signaling Regulates the Adaptive Responses of Aspergillus fumigatus to Thermal Stress and Other Related Stress
HOG-MAPK Signaling Regulates the Adaptive Responses of Aspergillus fumigatus to Thermal Stress and Other Related Stress
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DOI：
10.1007/s11046-012-9557-4
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发表时间：
2012-10-01
期刊：
影响因子：
5.5
通讯作者：
Li, Ruoyu
中科院分区：
文献类型：
--
作者：
Ji, Yajuan;Yang, Fang;Li, Ruoyu
烟曲霉自然暴露于高度可变的环境中，并受到各种胁迫。高渗甘油丝裂原活化蛋白激酶（HOG-MAPK）通路在调节细胞内环境变化的稳态中起着重要作用。本文探讨了HOG-MAPK通路在热胁迫及其他相关胁迫适应性反应中的作用。烟熏。我们在37和48 A摄氏度下观察了野生型菌株及其衍生突变体的表型特征，结果表明tcsB参与了高温反应。抗真菌药敏试验表明，SHO 1分支可能参与了A.烟曲霉对伊曲康唑的敏感性。热胁迫下野生型AF 293 sakA mRNA表达水平无明显变化，但在冷胁迫、250 mmol/L制霉菌素或10%二甲基亚砜处理下，磷酸化SakAp表达水平显著升高，且与SLN 1分支有关，与SHO 1分支无关。这些结果表明HOG-MAPK通路，尤其是SLN 1分支，在A.烟曲霉对热胁迫和其他相关胁迫的响应。
Aspergillus fumigatus is naturally exposed to a highly variable environment and subjected to various kinds of stresses. High-osmolarity glycerol mitogen-activated protein kinase (HOG-MAPK) pathway plays a crucial role in regulating cellular homeostasis in response to environmental changes. Here, we explored the contribution of HOG-MAPK pathway to the adaptive responses to thermal stress and other related stresses in A. fumigatus. We observed the phenotype features of wild-type strains and their derived mutants at 37 and 48 A degrees C, and the results suggested that tcsB participates in response to high temperature. Furthermore, susceptibility test for antifungal drugs showed that SHO1 branch is probably involved in the susceptibility of A. fumigatus to itraconazole at high temperature. Although sakA expression at mRNA level appeared unchanged in wild-type AF293 subjected to thermal stress, phosphorylated SakAp level increased significantly in the strains exposed to cold stress, 250 mmol/L nystatin or 10 % dimethyl sulfoxide in a manner dependent on the SLN1 branch and independent on the SHO1 branch. Taken together, these results indicate that HOG-MAPK pathway, especially the SLN1 branch, plays an important role in the adaptations of A. fumigatus to thermal stress and other related stresses.