Brachycoelium salamandrae (Frolich, 1789) (Digenea: Brachycoeliidae): Ultrastructural study of spermiogenesis and the mature spermatozoon
Brachycoelium salamandrae (Frolich, 1789) (Digenea: Brachycoeliidae): Ultrastructural study of spermiogenesis and the mature spermatozoon
复制标题
DOI：
10.1016/j.jcz.2012.05.003
复制
发表时间：
2013-01-01
影响因子：
1.4
通讯作者：
Miquel, Jordi
中科院分区：
文献类型：
--
作者：
Bakhoum, Abdoulaye J. S.;Ribas, Alexis;Miquel, Jordi
本文用透射电镜观察了银鱼精子发生和成熟精子的超微结构特征。精子发生开始于一个分化区的形成，该分化区包含一个细胞核、几个线粒体和两个中心粒，中心粒与横纹细根相连，中心粒间体位于它们之间。两个中心粒起源于两个游离鞭毛，在与中间胞质突融合之前，鞭毛垂直于中间胞质突生长。精子发生的最后阶段以弓形膜环的收缩和年轻精子的释放为特征。在这最后阶段，梨形的电子致密物质出现在中心粒的水平。B精子。蝾螈具有两个不同长度的轴丝，具有9+“1”trepaxonematan模式，一个细胞核，两个线粒体，两束平行的皮质微管和糖原颗粒。此外，B.蝾螈显示质膜和刺状体的外部结构。与B最相关的精子性状。并与其他属于Gorgoderoidea的物种进行了比较。(C)2012 Elsevier GmbH. All rights reserved.
Ultrastructural characters of spermiogenesis and the mature spermatozoon of Brachycoelium salamandrae are described by means of transmission electron microscopy. Spermiogenesis begins with the formation of a differentiation zone containing a nucleus, several mitochondria, and two centrioles associated with striated rootlets and with an intercentriolar body between them. The two centrioles originate two free flagella that grow orthogonally to the median cytoplasmic process before fusing with it. The final stage of spermiogenesis is characterized by a constriction of the ring of arched membranes and the liberation of the young spermatozoon. In this final stage a pear shaped electron-dense material appears at the level of centrioles. Spermatozoon of B. salamandrae exhibits two axonemes of different length with the 9+'1' trepaxonematan pattern, a nucleus, two mitochondria, two bundles of parallel cortical microtubules and granules of glycogen. Additionally, the spermatozoon of B. salamandrae shows external ornamentations of the plasma membrane and spinelike bodies. The most relevant spermatozoon characters of B. salamandrae are discussed and compared with those of other species belonging to the superfamily Gorgoderoidea. (C) 2012 Elsevier GmbH. All rights reserved.