Simulating gastrulation development and germ cell fate in vitro using human and monkey pluripotent stem cells
Simulating gastrulation development and germ cell fate in vitro using human and monkey pluripotent stem cells
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使用人和猴多能干细胞在体外模拟原肠胚发育和生殖细胞命运
DOI：
10.1038/protex.2017.050
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Kobayashi T
中科院分区：
文献类型：
--
作者：
Kobayashi T
该方案描述了一个使用多能干细胞（hPSCs）的体外模型，用于早期人类发育模拟导致原肠胚形成的关键事件。原始生殖细胞（PGCs）分化能力的获得和丧失发生在中胚层（ME）分化过程中，中胚层是最终胚层（DE）和中胚层的共同前体。该模型同样适用于食蟹猴PSCs （cmPSCs）。这些观察结果表明外胚层的原肠胚周围发育是保守的。该方案适用于灵长类动物早期移植后发育和早期细胞命运决定机制的研究。
This protocol describes an in vitro model using pluripotent stem cells (hPSCs), for early human development simulating key events leading to gastrulation. Gain and loss of competency for the specification of primordial germ cells (PGCs) occurs in the course of mesendoderm (ME) differentiation, a common precursor for definitive endoderm (DE) and mesoderm. This model is also applicable to cynomolgus monkey PSCs (cmPSCs). The observations suggest conserved principle for peri-gastrulation development of the epiblast. The protocol is suitable for investigations relating to early postimplantation development and mechanisms of early cell fate decisions in primates.