Novel in vitro comparative model of osteogenic and inflammatory cell response to dental implants
Novel in vitro comparative model of osteogenic and inflammatory cell response to dental implants
复制标题
DOI：
10.1016/j.dental.2018.11.011
复制
发表时间：
2019-01-01
期刊：
影响因子：
5
通讯作者：
Olivares-Navarrete, Rene
中科院分区：
文献类型：
--
作者：
Hotchkiss, Kelly M.;Sowers, Kegan T.;Olivares-Navarrete, Rene
目标。粗化牙种植体能促进间充质干细胞(MSCs)向成骨细胞分化，亲水性修饰可诱导抗炎巨噬细胞活化。虽然不同的表面修饰对商业牙种植体的骨整合的影响已经在体内和临床上进行了比较，但对这些修饰的初始细胞反应往往被忽视。我们的目标是在体外研究不同商业植入物对巨噬细胞炎症反应和MSC成骨的影响。研究人员检查了6种商用粗制植入物[OsseoSpeedTM(TM)(Astra-Tech(TM)，植入体A)；Osseotte(R)(Biomet 3i(TM)，植入体B)；TiUnite(TM)(诺贝尔生物保健(R)，植入体C)；Ti-SLA(注册商标)，(植入体D)，Roxolid(注册商标)(RXD-SLA，植入体E)，RXD-SLActive(注册商标)(植入体F)(Shurann(注册商标))]。巨噬细胞和间充质干细胞直接种植在种植体上，并在定制的培养瓶中培养。分别于24 h和48 h检测巨噬细胞前炎症标志物(IL1B、IL6、IL17A、CXCL10、TNFa)和抗炎症标志物(IL4、IL10、TGFB1)的mRNA和蛋白水平。7天后用碱性磷酸酶活力、骨钙素、血管生成、成骨和炎症标记物通过酶联免疫吸附试验和定量聚合酶链式反应(n=6/Variable，ANOVA，After Tukey HSD，α=0.05)鉴定MSCs的成骨分化。亲水性植入物F诱导MSCs释放的成骨因子水平最高，巨噬细胞释放的抗炎因子水平最低。或者，植入物A和C支持较低的成骨水平和增加促炎因子的分泌。在这项研究中，我们使用体外模型成功地评估了细胞对商用临床植入物的反应差异。来自该模型的数据表明，并不是所有的表面修饰过程都会产生相同的细胞反应。(C)2018年提交人(S)。由爱思唯尔公司出版。这是CC BY-NC-ND许可证(http://creativecommons.org/licenses/by-nc-nd/4.0/)下的一篇开放获取文章
Objectives. Roughened dental implants promote mesenchymal stem cell (MSCs) osteoblastic differentiation, and hydrophilic modifications induce anti-inflammatory macrophages activation. While the effect of different surface modifications on osseointegration of commercial dental implants have been compared in vivo and clinically, the initial cellular response to these modifications often overlooked. We aimed to characterize the macrophage inflammatory response and MSC osteogenesis across different commercially available implants in vitro.Methods. Six commercially available rough implants [OsseoSpeed (TM) (Astra-Tech (TM), Implant A); Osseotite (R) (Biomet 3i (TM), Implant B); TiUnite (TM) (Nobel-Biocare (R), Implant C); Ti-SLA (R), (Implant D), Roxolid (R) (RXD-SLA, Implant E), RXD-SLActive (R) (Implant F) (Straumann (R))] were examined. Macrophages and MSCs were seeded directly on implants and cultured in custom vials. mRNA and protein levels of pro- (IL1B, IL6, IL17A, CXCL10, TNFa) and anti- (IL4, IL10, TGFB1) inflammatory markers were measured after 24 and 48 h in macrophages. Osteoblastic differentiation of MSCs was assessed after seven days by alkaline phosphatase activity, osteocalcin, and angiogenic, osteogenic, and inflammatory markers by ELISA and qPCR (n = 6/variable, ANOVA, post hoc Tukey HSD with alpha = 0.05).Results. Hydrophilic implant F induced the highest level of osteogenic factor released from MSCs and anti-inflammatory factors from macrophages with the lowest level of pro-inflammatory factors. Alternatively, implants A and C supported lower levels of osteogenesis and increased secretion of pro-inflammatory factors.Significance. In this study, we successfully evaluated differences in cell response to commercially available clinical implants using an in vitro model. Data from this model suggest that not all surface modification procedures generate the same cell response. (C) 2018 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)