Novel capsaicin (VR1) and purinergic (P2X3) receptors in Hirschsprung's intestine
Novel capsaicin (VR1) and purinergic (P2X3) receptors in Hirschsprung's intestine
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DOI：
10.1053/jpsu.2001.27959
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发表时间：
2001-11-01
影响因子：
2.4
通讯作者：
Anand, P
中科院分区：
文献类型：
--
作者：
Facer, P;Knowles, CH;Anand, P
背景/目的：对先天性巨结肠症（HSCR）的研究表明，无神经节肠中的肥大神经主要是外源性的，可能含有感觉成分。最近的研究表明，辣椒素受体VR 1方法：本研究首次采用免疫组织化学方法，对正常成人、婴儿和HSCR大肠组织中VR 1和P2 X（3）免疫反应性的分布进行了研究。VR 1-免疫反应纤维和神经束，但不是胞体，检测到在所有区域的肠粘膜/固有层中的几个弱免疫染色纤维的对照。hypoganglionic和aganglionic肠肥大神经束表现出强烈的VR 1免疫反应，而normoganglionic地区的HSCR与对照组相似。在对照组婴儿、成人和神经节HSCR样本的肌间和粘膜下神经丛中检测到P2 X（3）免疫反应性神经元细胞体，在某些情况下具有长轴突突起。结论：无神经节的HSCR肠组织中存在VR 1和P2 X（3）免疫反应，提示感觉神经可能在其肥大神经支配中占很大比例。P2 X（3）和VR 1受体在肠神经中的功能意义值得进一步研究。小儿外科杂志36：1679-1684。Copyright（C）2001 by W.B.桑德斯公司
Background/Purpose: Studies of Hirschsprung's disease (HSCR) have shown that hypertrophic nerves in aganglionic bowel are mainly of extrinsic origin and may contain sensory elements. Recent advances have shown a specific capsaicin receptor VR1 (vanilloid receptor-1), and an ATP-gated ion channel P2X(3), which are expressed by sensory neurons.Methods: This study investigated, for the first time, the distribution of VR1- and P2X(3)-immunoreactivity in normal adult, infant, and HSCR large intestine, using specific antibodies for immunohistochemistry.Results: VR1-immunoreactive fibers and nerve fascicles, but not somata, were detected in all regions of the bowel in controls with few weakly immunostained fibers in the mucosa/ lamina propria. Hypertrophic nerve bundles in hypoganglionic and aganglionic bowel showed intense VR1-immunoreactivity, whereas normoganglionic regions of HSCR were similar to controls. P2X(3)-immunoreactive neuronal cell bodies, in some instances with long axonal processes, were detected in the myenteric and submucous plexuses in control infant, adult, and ganglionic HSCR samples. Aganglionic samples showed weak P2X(3)-immunoreactivity in hypertrophic nerve fasciculi in the submucous and myenteric plexuses.Conclusions: The presence of VR1- and P2X(3)-immunoreactivities in aganglionic HSCR bowel indicates that sensory nerves may form a significant proportion of its hypertrophic innervation. The functional significance of P2X(3) and VR1 receptors in enteric nerves deserves further investigation. J Pediatr Surg 36:1679-1684. Copyright (C) 2001 by W.B. Saunders Company.