Tyrosinase gene expression in zebrafish embryos
Tyrosinase gene expression in zebrafish embryos
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DOI：
10.1007/s004270000125
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发表时间：
2001-03-01
影响因子：
2.4
通讯作者：
Lardelli, M
中科院分区：
文献类型：
--
作者：
Camp, E;Lardelli, M
酪氨酸酶是酪氨酸转化为黑色素所必需的。因此，酪氨酸酶基因的表达是研究胚胎发生过程中表达黑色素细胞分化的一个有用的标志。我们描述了斑马鱼全胚中酪氨酸酶基因转录的时空模式和活性酶的存在。在受精后16.5小时，酪氨酸酶基因在发育中的视网膜色素上皮的背端转录，大约在可见色素沉着前7小时。此后不久，神经嵴源性黑素细胞的转录首先在中脑、间脑和后脑后/脊髓前侧的背侧被观察到。然后观察到基因激活和细胞迁移的波向动物的后部移动。酪氨酸酶活性的DOPA染色显示，在可见色素沉着前至少3小时，胚胎中就存在酶活性。
The enzyme tyrosinase is required for the conversion of tyrosine into the pigment melanin. Thus, tyrosinase gene expression is a useful marker for studying the differentiation of melanin-expressing cells during embryogenesis. We describe the spatiotemporal pattern of transcription of the tyrosinase gene and the presence of active enzyme in whole embryos of the zebrafish, Danio rerio. At 16.5 h post-fertilisation the tyrosinase gene is transcribed in the dorsal extremity of the developing retinal pigment epithelium, approximately 7 h before visible pigmentation. Shortly thereafter, transcription in neural crest-derived melanocytes is first observed dorsolateral to the mesencephalon and diencephalon and the posterior hindbrain/anterior spinal cord. A wave of gene activation and cell migration is then observed moving towards the posterior of the animal. DOPA staining for tyrosinase activity shows the presence of active enzyme in embryos at least 3 h before visible pigmentation.