Dissecting yield-associated loci in super hybrid rice by resequencing recombinant inbred lines and improving parental genome sequences
Dissecting yield-associated loci in super hybrid rice by resequencing recombinant inbred lines and improving parental genome sequences
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DOI：
10.1073/pnas.1306579110
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发表时间：
2013-08-27
影响因子：
11.1
通讯作者：
Qian, Qian
中科院分区：
文献类型：
--
作者：
Gao, Zhen-Yu;Zhao, Shan-Cen;Qian, Qian
世界人口的增长和可耕地面积的减少要求水稻作为最重要的粮食作物之一提高产量。为了阐明水稻产量的遗传基础并揭示其相关基因座，我们对广泛种植的超级杂交稻两优培九核心重组自交系进行了重测序，并构建了一张高分辨率的连锁图谱。共检测到43个与产量相关的数量性状基因座，其中20个是唯一的。在高密度物理图谱的基础上，两优培九的父本93-11和母本PA 64 s的基因组序列得到了显著改进。大量的重组自交系群体结合丰富的高质量的单核苷酸多态性和插入/缺失亲本基因组之间允许我们精细定位两个数量性状位点，qSN 8和qSPB 1，并确定抽穗天数8和疏松穗1作为候选基因，分别。数量性状位点qSN 8通过互补试验进一步确认为抽穗前天数8。本研究为水稻产量相关位点的分子育种提供了一个理想的平台。
The growing world population and shrinkage of arable land demand yield improvement of rice, one of the most important staple crops. To elucidate the genetic basis of yield and uncover its associated loci in rice, we resequenced the core recombinant inbred lines of Liang-You-Pei-Jiu, the widely cultivated super hybrid rice, and constructed a high-resolution linkage map. We detected 43 yield-associated quantitative trait loci, of which 20 are unique. Based on the high-density physical map, the genome sequences of paternal variety 93-11 and maternal cultivar PA64s of Liang-You-Pei-Jiu were significantly improved. The large recombinant inbred line population combined with plentiful high-quality single nucleotide polymorphisms and insertions/deletions between parental genomes allowed us to fine-map two quantitative trait loci, qSN8 and qSPB1, and to identify days to heading8 and lax panicle1 as candidate genes, respectively. The quantitative trait locus qSN8 was further confirmed to be days to heading8 by a complementation test. Our study provided an ideal platform for molecular breeding by targeting and dissecting yield-associated loci in rice.