Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins.
Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins.
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肺炎克雷伯菌的ntrC和nifA的序列和结构域关系：与其他调节蛋白的同源性。
DOI：
10.1002/j.1460-2075.1986.tb04230.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
J. Wootton
中科院分区：
文献类型：
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作者：
M. Drummond;P. Whitty;J. Wootton
我们已经确定了来自肺炎克雷伯氏菌的两个基因的核苷酸序列，nifA，nif特异性转录激活因子和ntrC，参与“氮控制”的双功能调节蛋白。这些序列与以前发表的序列有很大不同。特别地，nifA在先前报道的终止密码子之外延伸40个密码子。该延伸编码与苜蓿根瘤菌nifA蛋白高度同源的推定DNA结合结构域，并且在一定程度上与ntrC蛋白高度同源。在所有三种蛋白质中，该结构域通过可变长度的片段连接到240个残基的高度保守的中心结构域。具有结构域间接头性质的短片段将中心区域连接到N末端结构域，这在两种nifA蛋白的情况下是弱相关的。ntrC的N-末端结构域不具有这种同源性，这显然但出乎意料地与一组不同的原核多效性对照蛋白的N-末端结构域相关，包括来自大肠杆菌的ompR、dye和nusA以及来自枯草芽孢杆菌的spoOA和spoOF。
We have determined the nucleotide sequences of two genes from Klebsiella pneumoniae, nifA, the nif‐specific activator of transcription and ntrC, the bifunctional regulatory protein involved in ‘nitrogen control’. These sequences differ significantly from those previously published. In particular, nifA extends 40 codons beyond the stop codon reported earlier. This extension encodes a putative DNA‐binding domain strongly homologous to the Rhizobium meliloti nifA protein and to some extent to the ntrC protein. In all three proteins this domain is linked by a segment of variable length to a strongly conserved central domain of 240 residues. A short segment having the properties of an interdomain linker joins the central region to an N‐terminal domain, which is weakly related in the case of the two nifA proteins. This homology is not shared by the N‐terminal domain of ntrC, which is clearly but unexpectedly related to the N‐terminal domains of a diverse set of procaryotic pleiotropic control proteins, including ompR, dye and nusA from Escherichia coli and spoOA and spoOF from Bacillus subtilis.