Participation of the humoral immune system in the myeloma-specific transplantation resistance.
Participation of the humoral immune system in the myeloma-specific transplantation resistance.
复制标题
体液免疫系统参与骨髓瘤特异性移植抵抗。
DOI：
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发表时间：
1978
影响因子：
4.4
通讯作者：
Sandra H. Bridges
中科院分区：
文献类型：
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作者：
Sandra H. Bridges
研究报告，检查参与骨髓瘤特异性移植抵抗诱导的BALB/c小鼠与纯化的骨髓瘤蛋白M315预免疫的体液和细胞反应。使用浆细胞瘤脾集落形成试验定量评估接受M315免疫4 - 7次小鼠的未分级脾细胞或血清的受体的肿瘤免疫力。主要结果为：1）如果给予过继宿主M315加强免疫，则肿瘤免疫可以通过免疫脾细胞转移; 2）含有抗M315独特型抗体（a-Id 315）的被动转移血清也可以抑制肿瘤生长。在微量a-Id 315存在下，主动转移是成功的，而被动转移需要相对大量的活性。被动转移实验涉及延长的注射时间表，因此无法区分抗体或独特型特异性体液物质对肿瘤细胞的直接作用或对细胞免疫系统的诱导作用。检查参与移植免疫的免疫元件的性质的实验表明，一旦建立，它们对急性辐射（至800 R）和可的松损伤具有抗性。
Studies are reported that examine the participation of humoral and cellular responses in the myeloma-specific transplantation resistance induced by preimmunization of BALB/c mice with purified myeloma protein M315. A plasmacytoma spleen colony-forming assay was used to provide a quantitative estimate of the tumor immunity in recipients of unfractionated spleen cells or serum from mice immunized four to seven times with M315. The primary findings were: 1) that the tumor immunity can be transferred by immune spleen cells provided that a boost of M315 is given to adoptive hosts, and 2) that passively transferred serum containing anti-M315 idiotype antibody (a-Id315) can also inhibit tumor growth. Adoptive transfer was successful in the presence of minute amounts of a-Id315, whereas passive transfer required relatively large amounts of activity. The passive transfer experiments involved an extended injection schedule and thus could not discriminate between direct effects of antibody or idiotype-specific humoral substances on tumor cells or inductive effects on the cellular immune system. Experiments examining the properties of the immune elements involved in the transplantation immunity demonstrated that, once established, they are resistant to acute radiation (to 800 R) and cortisone damage.