PI4Kγ2 Interacts with E3 Ligase MIEL1 to Regulate Auxin Metabolism and Root Development1[OPEN]
PI4Kγ2 Interacts with E3 Ligase MIEL1 to Regulate Auxin Metabolism and Root Development1[OPEN]
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DOI：
10.1104/pp.20.00799
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发表时间：
2020-08
期刊：
影响因子：
7.4
通讯作者：
Chunzao Zhao;Hong-Wei Xue
中科院分区：
文献类型：
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作者：
Chunzao Zhao;Hong-Wei Xue
拟南芥磷脂酰肌醇激酶通过与E3连接酶相互作用并稳定E3连接酶来调节植物生长和抗病性，为E3在翻译后水平的调控提供了线索。根的发育对于植物的正常生长和养分吸收是重要的。研究揭示了各种因素参与这一复杂过程，提高了我们对相关调控机制的理解。在这里，我们功能性地描述了拟南芥（Arabidopsis thaliana）磷脂酰肌醇4-激酶γ2（PI 4K γ2）在根伸长调节中的作用，其功能是调节RING型E3连接酶MYB 30-交互E3连接酶1（MIEL 1）和生长素代谢的稳定性。PI 4K γ2（pi 4k γ2）缺失的突变体植株由于生长素水平降低而表现出根长缩短和伸长区缩短。PI 4K γ2与MIEL 1相互作用，调节其降解并促进转录因子MYB 30的稳定性（MYB 30通过直接结合GH3.2和GH3.6的启动子区域来抑制生长素代谢）。有趣的是，PI 4K γ2植物表现出改变的过敏反应，表明PI 4K γ2通过调节生长素代谢来调节植物的协同生长和防御。这些结果揭示了蛋白质相互作用在调节真核细胞中泛素介导的蛋白质降解中的重要性，并阐明了协调植物生长和生物胁迫反应的机制。
An Arabidopsis phosphatidylinositol kinase regulates plant growth and disease resistance through interacting with and stabilizing an E3 ligase, providing clues how E3 is regulated at post-translational level. Root development is important for normal plant growth and nutrient absorption. Studies have revealed the involvement of various factors in this complex process, improving our understanding of the relevant regulatory mechanisms. Here, we functionally characterize the role of Arabidopsis (Arabidopsis thaliana) phosphatidylinositol 4-kinase γ2 (PI4Kγ2) in root elongation regulation, which functions to modulate stability of the RING-type E3 ligase MYB30-INTERACTING E3 LIGASE1 (MIEL1) and auxin metabolism. Mutant plants deficient in PI4Kγ2 (pi4kγ2) exhibited a shortened root length and elongation zone due to reduced auxin level. PI4Kγ2 was shown to interact with MIEL1, regulating its degradation and furthering the stability of transcription factor MYB30 (which suppresses auxin metabolism by directly binding to promoter regions of GH3.2 and GH3.6). Interestingly, pi4kγ2 plants presented altered hypersensitive response, indicating that PI4Kγ2 regulates synergetic growth and defense of plants through modulating auxin metabolism. These results reveal the importance of protein interaction in regulating ubiquitin-mediated protein degradation in eukaryotic cells, and illustrate a mechanism coordinating plant growth and biotic stress response.