EXACERBATION OF MURINE LISTERIOSIS BY A MONOCLONAL-ANTIBODY SPECIFIC FOR THE TYPE-3 COMPLEMENT RECEPTOR OF MYELOMONOCYTIC CELLS - ABSENCE OF MONOCYTES AT INFECTIVE FOCI ALLOWS LISTERIA TO MULTIPLY IN NONPHAGOCYTIC CELLS
EXACERBATION OF MURINE LISTERIOSIS BY A MONOCLONAL-ANTIBODY SPECIFIC FOR THE TYPE-3 COMPLEMENT RECEPTOR OF MYELOMONOCYTIC CELLS - ABSENCE OF MONOCYTES AT INFECTIVE FOCI ALLOWS LISTERIA TO MULTIPLY IN NONPHAGOCYTIC CELLS
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DOI：
10.1084/jem.170.1.27
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发表时间：
1989-07-01
影响因子：
15.3
通讯作者：
NORTH, RJ
中科院分区：
文献类型：
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作者：
ROSEN, H;GORDON, S;NORTH, RJ
用对骨髓单核细胞的3型补体受体的表位具有特异性的大鼠mAb（5C6）处理小鼠严重干扰小鼠抵抗单核细胞增生李斯特菌感染的能力。因此，亚致死性感染迅速转化为致死性感染，导致在5天内死亡。然而，如果在感染早期给予5C6，在单核吞噬细胞占据肝脏和脾脏中李斯特菌植入部位之前，感染才会加重。如果在感染第3天后给药，5C6仅引起细菌增殖的暂时增加。5C6的感染增强作用与小鼠未能将单核吞噬细胞聚集在肝细胞中李斯特菌细菌增殖的位点和脾细胞外李斯特菌细菌增殖的位点有关。这导致李斯特菌在肝细胞和脾细胞外不受限制地增殖。结果与先前研究所揭示的5C6抑制腹膜炎性渗出物中骨髓单核细胞积聚的能力一致（5）。
Treatment of mice with a rat mAb (5C6) specific for an epitope of the type 3 complement receptor of myelomonocytic cells severely interfered with the ability of the mice to resist infection with Listeria monocytogenes. Consequently, a sublethal infection was rapidly converted to a lethal one that resulted in death in 5 d. However, infection was only exacerbated if 5C6 was given earlier in infection, before mononuclear phagocytes populated sites of Listeria implantation in the liver and spleen. If given after day 3 of infection, 5C6 caused only a temporary increase in bacterial multiplication. The infection-enhancing effect of 5C6 was associated with failure of mice to focus mononuclear phagocytes at sites of bacterial multiplication of Listeria in liver hepatocytes and extracellularly in the spleen. This resulted in unrestricted multiplication of Listeria in hepatocytes and extracellularly in the spleen. The results are in keeping with the ability of 5C6 to inhibit the accumulation of myelomonocytic cells in peritoneal inflammatory exudates, as revealed by a previous study (5).