Development and validation of functional kompetitive allele-specific PCR markers for herbicide resistance in Brassica napus
Development and validation of functional kompetitive allele-specific PCR markers for herbicide resistance in Brassica napus
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DOI：
10.3389/fpls.2023.1213476
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
赵坚义
中科院分区：
文献类型：
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作者：
石江华;余华胜;傅鹰;王枟刘;张尧峰;黄吉祥;李素娟;郑滔;倪西源;赵坚义
Effective weed control in the field is essential for maintaining favorable growing.conditions and rapeseed yields. Sulfonylurea herbicides are one kind of most.widely used herbicides worldwide, which control weeds by inhibiting.acetolactate synthase (ALS). Molecular markers have been designed from.polymorphic sites within the sequences of ALS genes, aiding marker-assisted.selection in breeding herbicide-resistant rapeseed cultivars. However, most of.them are not breeder friendly and have relatively limited application due to.higher costs and lower throughput in the breeding projects. The aims of this.study were to develop high throughput kompetitive allele-specific PCR (KASP).assays for herbicide resistance. We first cloned and sequenced BnALS1 and.BnALS3 genes from susceptible cultivars and resistant 5N (als1als1/als3als3.double mutant). Sequence alignments of BnALS1 and BnALS3 genes for.cultivars and 5N showed single nucleotide polymorphisms (SNPs) at positions.1676 and 1667 respectively. These two SNPs for BnALS1 and BnALS3 resulted in.amino acid substitutions and were used to develop a KASP assay. These.functional markers were validated in three distinct BC1F2 populations. The.KASP assay developed in this study will be valuable for the high-throughput.selection of elite materials with high herbicide resistance in rapeseed.breeding programs.
