Identification of extracellular vesicles and characterization of miRNA expression profiles in human blastocoel fluid
Identification of extracellular vesicles and characterization of miRNA expression profiles in human blastocoel fluid
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DOI：
10.1038/s41598-018-36452-7
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发表时间：
2019-01-14
影响因子：
4.6
通讯作者：
Di Pietro, C.
中科院分区：
文献类型：
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作者：
Battaglia, R.;Palini, S.;Di Pietro, C.
在这项研究中，我们首次证明了人类囊胚腔液中存在microRNA和细胞外囊泡。生物信息学和比较分析确定了囊胚腔液microRNA的生物学功能，并提出了人类囊胚内的潜在作用。我们发现了89种以不同水平表达的microRNA，它们能够调节控制胚胎发育的关键信号通路，如多能性、细胞重编程、表观遗传修饰、细胞间通讯、细胞粘附和细胞命运。囊胚腔液microRNA反映了胚胎细胞的miRNome，并且它们的存在与囊胚腔液内细胞外囊泡的发现相关，强烈表明它们在介导囊胚细胞之间的细胞通讯中的重要作用。它们的特征对于更好地理解胚胎发生的最早阶段和调节多能性的复杂电路是重要的。此外，囊胚腔液microRNA谱可能受到囊胚质量的影响，因此，microRNA可能用于评估IVF周期中的胚胎潜力。
In this study, for the first time, we demonstrated the presence of microRNAs and extracellular vesicles in human blastocoel fluid. The bioinformatic and comparative analyses identified the biological function of blastocoel fluid microRNAs and suggested a potential role inside the human blastocyst. We found 89 microRNAs, expressed at different levels, able to regulate critical signaling pathways controlling embryo development, such as pluripotency, cell reprogramming, epigenetic modifications, intercellular communication, cell adhesion and cell fate. Blastocoel fluid microRNAs reflect the miRNome of embryonic cells and their presence, associated with the discovery of extracellular vesicles, inside blastocoel fluid, strongly suggests their important role in mediating cell communication among blastocyst cells. Their characterization is important to better understand the earliest stages of embryogenesis and the complex circuits regulating pluripotency. Moreover, blastocoel fluid microRNA profiles could be influenced by blastocyst quality, therefore, microRNAs might be used to assess embryo potential in IVF cycles.