Cloning and sequencing of a novel human gene that encodes a putative target protein of Nesh-SH3
Cloning and sequencing of a novel human gene that encodes a putative target protein of Nesh-SH3
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DOI：
10.1007/s100380170049
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发表时间：
2001-01-01
影响因子：
3.5
通讯作者：
Hamaguchi, M
中科院分区：
文献类型：
--
作者：
Matsuda, S;Iriyama, C;Hamaguchi, M
通过使用常规的双杂交技术，以Nesh的Src同源性3（SH 3）结构域为诱饵蛋白，从人胎盘cDNA文库中分离并测序了一个新的全长cDNA。该cDNA全长3023 bp，开放阅读框编码486个氨基酸。它具有SH3结合基序，核靶向序列，没有催化结构域。总的来说，它与参与信号级联的已知分子没有相似性。使用单染色体杂交组和辐射杂交细胞组进行基于聚合酶Chair反应的作图，将该基因定位在人类染色体3q12上靠近标记D3S1271的位置。
By using a conventional two-hybrid technique with an Src homology 3 (SH3) domain of Nesh as the bait protein, a novel full-length cDNA was isolated and sequenced from a human placenta cDNA library. This cDNA consists of 3023bp and has a predicted open reading frame that encodes 486 amino acids. It possesses an SH3 binding motif, a nuclear targeting sequence, and no catalytic domain. Overall, it has no similarity to known molecules involved in a signaling cascade. Polymerase Chair reaction-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels localized the gene on human chromosome 3q12 near the marker D3S1271.