Interplay of Nanoparticle Resonance Frequency and Array Surface Coverage in Live-Cell Plasmon-Enhanced Single-Molecule Imaging
Interplay of Nanoparticle Resonance Frequency and Array Surface Coverage in Live-Cell Plasmon-Enhanced Single-Molecule Imaging
复制标题
活细胞等离子体增强单分子成像中纳米颗粒共振频率和阵列表面覆盖的相互作用
DOI：
10.1021/acs.jpcc.8b01436
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发表时间：
2018
影响因子：
3.7
通讯作者：
J. Biteen
中科院分区：
文献类型：
--
作者：
Stephen A. Lee;J. Biteen
超分辨率成像为纳米级光学提供了新的见解。利用纳米球光刻技术制备的等离子体金纳米三角形阵列可以增强单分子荧光强度，从而进一步提高成像效果。在这里，玻璃盖上的金纳米三角形阵列被用作廉价、简单、广泛适用的成像底物，用于表达光激活荧光蛋白（红色PAmCherry或绿色pagfp）的活霍乱弧菌细胞，并在活细胞与纳米三角形阵列耦合后产生荧光增强。在这种宽场耦合几何结构的要求下，我们分析并优化了耦合作为局部表面等离子体共振频率和粒子覆盖率的函数。
Super-resolution imaging has provided new insights into nanoscale optics. Plasmonic gold nanotriangle arrays created by nanosphere lithography can enhance single-molecule fluorescence intensity to further improve imaging. Here, gold nanotriangle arrays on glass coverslips were used as inexpensive, facile, and broadly applicable imaging substrates for living Vibrio cholerae cells expressing photoactivatable fluorescent proteins—the red PAmCherry or the green PAGFP—and resulted in fluorescence enhancements upon coupling living cells to nanotriangle arrays. Within the requirements for this wide-field coupling geometry, we analyze and optimize the coupling as a function of local surface plasmon resonance frequency and particle coverage.