Near-infrared fluorescence imaging and photodynamic therapy with indocyanine green lactosomes has antineoplastic effects for gallbladder cancer.
Near-infrared fluorescence imaging and photodynamic therapy with indocyanine green lactosomes has antineoplastic effects for gallbladder cancer.
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DOI：
10.18632/oncotarget.27193
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发表时间：
2019-09-24
期刊：
影响因子：
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通讯作者：
Yoshii, Kengo
中科院分区：
文献类型：
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作者：
Hishikawa, Hidehiko;Kaibori, Masaki;Yoshii, Kengo
背景：利用吲哚菁绿(ICG)-乳小体研究近红外荧光（NIF）成像和光动力治疗（PDT）对胆囊癌的诊断和治疗效果。结果：PDT对icg -乳小体处理的NOZ细胞有毒性。注射后≥24小时，注射ICG-乳小体的小鼠肿瘤区域的荧光强度高于健康的对侧区域，而不是单独注射ICG。PDT对注射icg -乳小体的NOZ小鼠具有即时和持续的光毒性作用。与一次照射组相比，两次照射组的抗肿瘤作用增强。方法：将ICG或ICG-乳小体加入人胆囊癌细胞株NOZ后，采用PDT法测定细胞活力。PDT后进行ICG和ICG-乳小体质谱分析。将ICG或ICG-乳小体静脉注射到皮下植入NOZ细胞的BALB/c裸鼠，并通过NIF成像评价其荧光性。植入肿瘤行PDT， ig -乳小体组照射1次或2次后，观察其抗肿瘤效果。结论：在异种移植物肿瘤和PDT中积累的icg -乳小体对这些恶性肿瘤具有抗肿瘤作用。与icg -乳小体和PDT结合的NIF成像可能是胆囊癌的有用诊断和/或治疗药物。
BACKGROUND: The diagnostic use and therapeutic effect of near infrared fluorescence (NIF) imaging and photodynamic therapy (PDT) was investigated for gallbladder cancer using indocyanine green (ICG)-lactosomes. RESULTS: PDT was toxic for NOZ cells treated with ICG-lactosomes. Fluorescence intensity in the tumor region of mice administered ICG-lactosomes, but not ICG alone, was higher than the healthy contralateral region ≥24 hours after injection. PDT exerted immediate and continuous phototoxic effects in NOZ implanted mice injected with ICG-lactosomes. Enhanced antitumor effects were observed in the twice irradiated group compared with the once irradiated group. METHOD: ICG or ICG-lactosomes were added to the human gallbladder cancer cell line NOZ followed by PDT and cell viability was measured. Mass spectrometry of ICG and ICG-lactosomes was performed after PDT. ICG or ICG-lactosomes were intravenously administered to BALB/c nude mice implanted subcutaneously with NOZ cells and fluorescence was evaluated by NIF imaging. Implanted tumors underwent PDT and antitumor effects were analyzed after performing irradiation once or twice in ICG-lactosome groups. CONCLUSIONS: ICG-lactosomes accumulated in xenograft tumors and PDT had an antitumor effect on these malignant tumors. NIF imaging with ICG-lactosomes and PDT may be useful diagnostic and/or therapeutic agents for gallbladder cancer.