A novel synergistic enhanced chemiluminescence achieved by a multiplex nanoprobe for biological applications combined with dual-amplification of magnetic nanoparticles
A novel synergistic enhanced chemiluminescence achieved by a multiplex nanoprobe for biological applications combined with dual-amplification of magnetic nanoparticles
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DOI：
10.1039/c0sc00341g
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发表时间：
2010-01-01
期刊：
影响因子：
8.4
通讯作者：
Zhang, Shusheng
中科院分区：
文献类型：
--
作者：
Bi, Sai;Zhou, Hong;Zhang, Shusheng
基于磁性纳米粒子（MNPs）作为DNA分子载体和报告标记，并通过双重扩增（DNA扩增和金属离子扩增）进一步放大信号，将多重纳米探针（cu /DNA/Au/DNA/MNP）实现了一种新型协同增强化学发光（SECL）系统应用于信号扩增生物分析策略。本研究首次提出了一种鲁米诺- h2o2 -Cu2+-Fe3+ SECL体系，该体系由多组分纳米粒子探针实现，与单金属Cu2+或Fe3+催化剂相比，该体系明显增强了CL信号。SECL方法的显著灵敏度与MNPs的扩增相结合，实现了特异性DNA序列和Ramos细胞的检测，分别低至6.8 aM和56细胞mL(-1)，是生物检测中灵敏度最高的方法之一。此外，基于Ramos细胞适体的高特异性，该策略能够区分Ramos靶细胞和CEM对照细胞，表明该策略在病变细胞检测中具有广泛的适用性。
A multiplex nanoprobe (CuS/DNA/Au/DNA/MNP) achieving a novel synergistic enhanced chemiluminescence (SECL) system is applied in a signal amplified bioassay strategy based on magnetic nanoparticles (MNPs) acting as both DNA molecular carriers and reporter labels, and further amplifying the signals by dual-amplification (DNA amplification and metal ions amplification). In this study, a luminol-H2O2-Cu2+-Fe3+ SECL system is proposed for the first time, which is achieved by a multicomponent nanoparticle probe and significantly intensifies the CL signal compared to monometallic Cu2+ or Fe3+ catalyst. The combination of the remarkable sensitivity of the SECL method with the amplification of MNPs achieves the detection of specific DNA sequences and Ramos cells as low as 6.8 aM and 56 cells mL(-1), respectively, which is one of the most sensitive approaches for bioassays. In addition, the strategy is able to differentiate between Ramos target cells and CEM control cells based on the high specificity of the aptamer for Ramos cells, indicating the wide applicability of it for diseased cell detection.