SITE OF AIRWAY CLOSURE IN EXCISED DOG LUNGS - HISTOLOGIC DEMONSTRATIION
SITE OF AIRWAY CLOSURE IN EXCISED DOG LUNGS - HISTOLOGIC DEMONSTRATIION
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DOI：
10.1152/jappl.1970.29.3.340
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发表时间：
1970-01-01
影响因子：
3.3
通讯作者：
KIVITZ, PB
中科院分区：
文献类型：
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作者：
HUGHES, JMB;ROSENZWEIG, DY;KIVITZ, PB
方法灰狗静脉注射硫喷妥钠0.4- 0.6g麻醉，肝素2000 IU/kg。放血后，取出左肺，灌注并通气，如所述（4）。将肺水平放置在Lucite盒中。用2升钟形肺量计记录肺容量变化，用水压计记录经肺动脉压变化。气道压力保持在大气压;通过改变箱内压力来改变胸膜压力。未测量绝对肺容积。除非特别提及，否则所有测量均使用相同的体积历史。通过将箱中的压力降低至-24 cm Hz 0使肺最大程度地膨胀，然后从10至15 cm Hz 0经肺压（Ptp）通气3-4次。然后将肺从Ptp 5缓慢充气至21 cm HZO，并在肺放气至6 cm HZO的负Ptp时记录经肺动脉压和体积变化。在Ptp-2和-4 cm HZO之间停止排出气体。大多数制备物在37 ℃下用血液灌注。用盐水压力计测量肺动脉和静脉压，并通过在圆筒中收集定时样品来测量血流量。流量由滚子泵控制。如果需要灌注超过几分钟，则从杂种狗中采集稳定的静脉血，将离开离体肺的血液返回到杂种狗体内。在10项实验中，通过支气管插管将2-4 ml造影剂（油性或水性Dionosil）滴入气道。在另外三个实验中，如Leopold和Gough（7）所述，将细颗粒铅引入气道。在充气至Ptp 21 cm HZO后，在约5分钟内将肺缓慢放气至Ptp-6 cm HZO。在Ptp 21、10、5、0和-6 cm HZO处拍摄X线片。使用非屏幕胶片和0.3 mm焦点。X线源至肺部的距离为96 cm，肺部至胶片的距离为5-7 cm。
METHODSGreyhound dogs were anesthetized with intravenous thiopentone(0.4-0.6 g) and heparinized(2000 IU/kg). After exsanguination the left lungs were removed, perfused, and ventilated as described (4). Lungs were placed horizontally in a Lucite box. Volume changes were recorded on a 2-liter bell spirometer and transpulmonary pressure changes with a water manometer. Airway pressure remained at atmospheric; pleural pressure was changed by altering the pressure in the box. Absolute lung volume was not measured. Unless specifically mentioned the same volume history was used for all measurements. The lung was inflated maximally by lowering the pressure in the box to-24 cm Hz0 and then ventilated for 3-4 breaths from 10 to 15 cm Hz0 transpulmonary pressure (Ptp). The lung was next slowly inflated from Ptp 5 to 21 cm Hz0 and transpulmonary pressure and volume changes were recorded as the lung was deflated to a negative Ptp of 6 cm HZO. Expulsion of gas ceased between Ptp-2 and-4 cm HZO. Most of the preparations were perfused with blood at 37 C. Pulmonary arterial and venous pressures were measured with saline manometers, and blood flow by collection of timed samples in a cylinder. Flow was controlled by a roller pump. If perfusion was needed for longer than a few minutes a steady flow of venous blood was taken from a mongrel dog to which the blood leaving the isolated lung was returned.X-ray measurements. In 10 experiments 2-4 ml of contrast medium (oily or aqueous Dionosil) was instilled into the airways through the bronchial cannula. In three other experiments finely particulate lead was introduced into the airways as described by Leopold and Gough (7). After inflation to Ptp 21 cm HZO, the lung was deflated slowly over about 5 min to Ptp-6 cm HZO. Radiographs were taken at Ptp 21, 10, 5, 0, and-6 cm HZO. A nonscreen film and a 0.3-mm focal spot was used. The distance from the X-ray source to the lung was 96 cm, and from the lung to the film was 5-7 cm.