CELLS OF THE PERIRETICULAR NUCLEUS PROJECT TO THE DEVELOPING NEOCORTEX OF THE RAT
CELLS OF THE PERIRETICULAR NUCLEUS PROJECT TO THE DEVELOPING NEOCORTEX OF THE RAT
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DOI：
10.1002/cne.903590408
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发表时间：
1995-09-04
影响因子：
2.5
通讯作者：
BAKER, GE
中科院分区：
文献类型：
--
作者：
ADAMS, NC;BAKER, GE
网状周围核是最近描述的内囊纤维中的小细胞薄片，位于丘脑网状核外侧和苍白球内侧（Clemence和Mitrofanis [1992]. J. Comp.神经元322：167-180）。在发育过程中，网状周核相对较大，位于生长的离皮质和丘脑皮质轴突的路径上，并填充丘脑网状核外侧的内囊区域。在这些轴突形成它们的连接之后，网状周围核的大小迅速减小，在成体中仅留下少数细胞（Mitrofanis [1992] J.Comp.Neurol. 320：161-181）。在这项研究中，我们的目的是通过将示踪剂注射到皮质板中来研究发育中的皮质和丘脑之间的联系。用辣根过氧化物酶（HRP）、麦胚凝集素结合HRP（WGA-HRP）和1 ′-双十八烷基-3，3，3 ′，3-四甲基羰花青高氯酸盐（DiI）在胚胎（E）18天至成年的大鼠体内注射，并将DiI置于E16天至生后（P）1天的大鼠固定脑内。由这些注射产生的逆行核周标记揭示了从网状周围核到同侧皮质板的短暂投射。在丘脑网状核中没有标记细胞，这表明网状周核是一组与丘脑网状核不同的细胞，在发育过程中起着不同的作用。网状周围细胞和皮质亚板细胞之间的比较表明，两者可能在早期发育中发挥类似的作用，可能引导纤维走向其终点站或重新排列连接丘脑和皮质的复杂映射投影。(C)1995年Wiley-Liss，Inc.
The perireticular nucleus is a recently described thin sheet of small cells among the fibres of the internal capsule, lying lateral to the thalamic reticular nucleus and medial to the globus pallidus (Clemence and Mitrofanis [1992]. J. Comp. Neurol. 322:167-180). During development, the perireticular nucleus is relatively large, lying in the path of the growing corticofugal and thalamocortical axons and filling the area of the internal capsule lateral to the thalamic reticular nucleus. After these axons have formed their connections, the perireticular nucleus rapidly decreases in size, leaving only a few cells in the adult (Mitrofanis [1992] J. Comp. Neurol. 320:161-181).In this study, we aimed to investigate the connections between the developing cortex and thalamus by making injections of tracer into the cortical plate. Injections of Horse Radish Peroxidase (HRP), Wheat Germ Agglutinin bound to HRP (WGA-HRP) and 1'dioctadecyl-3,3,3',3 tetramethycarbocyanine perchlorate (DiI) were made in vivo between embryonic day (E)18 and adult and DiI was placed in the fixed brains of rats aged between E16 and postnatal day (P)1.Between E17 and P10, the retrograde perikaryal labelling resulting from these injections revealed a transient projection from the perireticular nucleus to the ipsilateral cortical plate. No cells were labelled in the thalamic reticular nucleus.This suggests that the perireticular nucleus must be regarded as a group of cells distinct from the thalamic reticular nucleus and having a separate role in development. Comparisons between the perireticular cells and the cells of the cortical subplate suggest that both may be playing comparable roles in early development, possibly guiding fibres towards their end stations or serving to rearrange the complex mapped projections linking thalamus and cortex. (C) 1995 Wiley-Liss, Inc.