The oncogenic role of EIF3D is associated with increased cell cycle progression and motility in prostate cancer
The oncogenic role of EIF3D is associated with increased cell cycle progression and motility in prostate cancer
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DOI：
10.1007/s12032-015-0518-x
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发表时间：
2015-07-01
期刊：
影响因子：
3.4
通讯作者：
Cui, Xingang
中科院分区：
文献类型：
--
作者：
Gao, Yi;Teng, Jingfei;Cui, Xingang
EIF 3是最大的多蛋白复合物，一些研究已经揭示了其亚基在许多人类癌症中的致癌作用。然而，EIF 3D在PCa的发展和进展中的作用仍然没有被发现。在本研究中，EIF 3D的表达在前列腺癌和癌旁组织，以及PCa细胞系，进行了检查。EIF 3D在PCa组织中的表达较癌旁组织明显上调。为了研究EIF 3D是否可以作为前列腺癌的潜在治疗靶点，EIF 3D被敲除以验证其在前列腺癌细胞中的功能作用。敲低EIF 3D后，PC-3和DU 145细胞的增殖、侵袭和集落形成均受到明显抑制，细胞周期分析显示细胞阻滞于G2/M期。EIF 3D与PCa相关，沉默EIF 3D将导致DU 145和PC-3细胞的增殖和迁移减少以及G2/M停滞。这些结果表明，EIF 3D在PCa的发展和进展中起致癌作用。
EIF3 is the largest multi-protein complex, and several studies have revealed the oncogenic roles of its subunits in many human cancers. However, the roles of EIF3D in the development and progression of PCa remain uncovered. In the present study, the expression of EIF3D in prostate cancer and paracarcinoma tissues, as well as PCa cell lines, was examined. In PCa tissues, the expression of EIF3D was up-regulated compared to that in paracarcinoma tissues. In order to investigate whether EIF3D could serve as potential therapeutic target for prostate cancer, EIF3D was knocked down to verify its functional role in prostate cancer cells. After EIF3D knockdown in PC-3 and DU145 cells, cell proliferation, invasion and colony formation were significantly inhibited; meanwhile, cell cycle analysis revealed cell cycle arrest at G2/M phase. EIF3D is associated with PCa, and silencing EIF3D will result in decreased proliferation, and migration, as well as G2/M arrest in DU145 and PC-3 cells. These results suggest that EIF3D plays an oncogenic role in PCa development and progression.