CARBOHYDRATES MEDIATE THE ADHERENCE OF HAMSTER SPERM TO OVIDUCTAL EPITHELIUM
CARBOHYDRATES MEDIATE THE ADHERENCE OF HAMSTER SPERM TO OVIDUCTAL EPITHELIUM
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DOI：
10.1095/biolreprod52.6.1395
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发表时间：
1995-06-01
影响因子：
3.6
通讯作者：
SUAREZ, SS
中科院分区：
文献类型：
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作者：
DEMOTT, RP;LEFEBVRE, R;SUAREZ, SS
哺乳动物输卵管的下峡部似乎是精子的储存器，精子通过粘附在上皮上而被保留。通过抑制精子结合在离体仓鼠输卵管，并利用碳水化合物探针，我们的特点是在水库的精子粘附，并建立了一个潜在的生化机制，精子的粘附和释放。胎球蛋白和它的末端糖，N-乙酰神经氨酸，干扰精子输卵管上皮的粘附。标记的胎球蛋白结合到新鲜附睾精子的顶体区域，但不是超活化精子，这是以前曾报道释放上皮结合。用胎球蛋白和唾液酸识别凝集素标记的蛋白质印迹鉴定了几种分子量的蛋白质，这些蛋白质是参与粘附的精子表面成分的候选者。这些候选人中的一些标记在来自超活化精子的样品中减少。这些结果表明，类似于胎球蛋白上发现的唾液酸化低聚糖可能被精子表面成分识别，并介导与输卵管上皮的结合。释放可以通过在获能或超活化期间组分的损失或修饰来实现。
The lower isthmus of the mammalian oviduct appears to serve as a reservoir for sperm that are retained by adherence to the epithelium. By inhibiting sperm binding within excised hamster oviducts and making use of carbohydrate probes, we have characterized the adherence of sperm in the reservoir and established a potential biochemical mechanism for the adherence and release of sperm. Fetuin and its terminal sugar, N-acetylneuraminic acid, interfered with the adherence of sperm to the oviductal epithelium. Labeled fetuin bound to the acrosomal region of fresh epididymal sperm, but not hyperactivated sperm, which have previously been reported to release from epithelial binding. Western blots labeled with fetuin and sialic acid-recognizing lectins identified proteins at several molecular masses that are candidates for the sperm surface component involved in adherence. Labeling of some of these candidates was reduced in samples from hyperactivated sperm. These results indicate that a sialylated oligosaccharide similar to that found on fetuin may be recognized by a sperm surface component and mediate binding to the oviductal epithelium. Release may be accomplished by loss or modification of the component during capacitation or hyperactivation.