A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP
A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP
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DOI：
10.1016/s0092-8674(00)00137-9
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发表时间：
2000-10-27
期刊：
影响因子：
64.5
通讯作者：
Lührmann, R
中科院分区：
文献类型：
--
作者：
Watkins, NJ;Ségault, V;Lührmann, R
盒C/D snoRNA在指导2 '-O-甲基化和/或作为核糖体RNA加工中的伴侣中起作用。我们在这里显示，Snu 13 p（在人类中为15.5kD），U4/U6.U5 tri-snRNP的一个组成部分，也与盒C/D snoRNA相关。事实上，酵母中Snu 13 p的遗传缺失导致RNA代谢的重大缺陷。盒C/D基序可以折叠成茎-内环-茎结构，与U4 snRNA中的15.5kD结合位点几乎相同。与此一致，盒C/D基序在体外结合Snu 13 p/15.5kD。在U4 snRNP（U6的伴侣）和盒C/D snoRNP之间观察到的结构和功能的相似性提出了有趣的可能性，即这些颗粒可能是从共同的祖先RNP进化而来的。
The box C/D snoRNAs function in directing 2'-O-methylation and/or as chaperones in the processing of ribosomal RNA. We show here that Snu13p (15.5kD in human), a component of the U4/U6.U5 tri-snRNP, is also associated with the box C/D snoRNAs. Indeed, genetic depletion of Snu13p in yeast leads to a major defect in RNA metabolism. The box C/D motif can be folded into a stem-internal loop-stem structure, almost identical to the 15.5kD binding site in the U4 snRNA. Consistent with this, the box C/D motif binds Snu13p/15.5kD in vitro. The similarities in structure and function observed between the U4 snRNP (chaperone for U6) and the box C/D snoRNPs raises the interesting possibility that these particles may have evolved from a common ancestral RNP.