Seroprevalence of Antibodies against Seal Influenza A(H10N7) Virus in Harbor Seals and Gray Seals from the Netherlands.
Seroprevalence of Antibodies against Seal Influenza A(H10N7) Virus in Harbor Seals and Gray Seals from the Netherlands.
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DOI：
10.1371/journal.pone.0144899
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kuiken T
中科院分区：
文献类型：
--
作者：
Bodewes R;Rubio García A;Brasseur SM;Sanchez Conteras GJ;van de Bildt MW;Koopmans MP;Osterhaus AD;Kuiken T
2014年春夏两季，在瑞典和丹麦沿海的斑海豹（Phoca vitulina）中爆发了海豹流感A（H10N7）病毒感染。这种病毒随后传播到德国和荷兰海岸的海豹身上。据报道，瑞典、丹麦和德国有数千只海豹死亡，而在荷兰，只有数量有限的海豹死亡。为了确定荷兰海豹暴露于流感A/H10N7病毒的程度，我们使用血凝抑制试验和酶联免疫吸附试验，测量了在荷兰捕获的活海豹和入院康复的海豹的血清样本中的特异性抗体滴度。在2015年的斑海豹中，有41%（78只幼海豹中的32只）、10%（52只断奶海豹中的5只）和58%（12只亚成年海豹或成年海豹中有7只）检测到海豹流感A（H10N7）病毒抗体。在2015年的灰海豹（Halichoerus grypus）中，幼崽（26只）中未发现特异性抗体，但在26%（19只中有5只）的老年动物中发现了特异性抗体。这些发现表明，尽管死亡率明显较低，但海豹流感A（H10N7）病毒感染在地理上广泛存在，也发生在灰海豹中。
In the spring and summer 2014, an outbreak of seal influenza A(H10N7) virus infection occurred among harbor seals (Phoca vitulina) off the coasts of Sweden and Denmark. This virus subsequently spread to harbor seals off the coasts of Germany and the Netherlands. While thousands of seals were reported dead in Sweden, Denmark and Germany, only a limited number of seals were found dead in the Netherlands. To determine the extent of exposure of seals in the Netherlands to influenza A/H10N7 virus, we measured specific antibody titers in serum samples from live-captured seals and seals admitted for rehabilitation in the Netherlands by use of a hemagglutination inhibition assay and an ELISA. In harbor seals in 2015, antibodies against seal influenza A(H10N7) virus were detected in 41% (32 out of 78) pups, 10% (5 out of 52) weaners, and 58% (7 out of 12) subadults or adults. In gray seals (Halichoerus grypus) in 2015, specific antibodies were not found in the pups (n = 26), but in 26% (5 out of 19) of the older animals. These findings indicate that, despite apparent low mortality, infection with seal influenza A(H10N7) virus was geographically widespread and also occurred in grey seals.