New Cleavable Spacers for Tandem Synthesis of Multiple Oligonucleotides
New Cleavable Spacers for Tandem Synthesis of Multiple Oligonucleotides
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DOI：
10.1055/a-1538-9883
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发表时间：
2021-06
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影响因子：
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通讯作者：
Y. Hari;Kazuki Yamamoto;Yasufumi Fuchi;Masaya Okabe;T. Ōsawa;Yuta Ito
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文献类型：
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作者：
Y. Hari;Kazuki Yamamoto;Yasufumi Fuchi;Masaya Okabe;T. Ōsawa;Yuta Ito
在固相寡核苷酸合成中，单个寡核苷酸通常是从装载特定固体载体的色谱柱中获得的。在此，我们开发了新的可切割间隔（CS）衍生物，用于在单柱上串联合成多个寡核苷酸。设计、合成了4个CS类似物，并使用自动寡核苷酸合成器将其插入两个寡核苷酸序列之间。含环顺式-1,2-二醇的CS衍生物在常用的氨水基本条件下表现出两种寡核苷酸的有效释放。在CS类似物中，具有较强结构的CS可作为间隔分子用于串联合成多个寡核苷酸。
Abstract In solid-phase oligonucleotide synthesis, a single oligonucleotide is generally acquired from a column loaded with a specific solid support. Herein, we have developed new cleavable spacer (CS) derivatives for tandem synthesis of multiple oligonucleotides on a single column. Four CS analogues were designed, synthesized, and inserted between two oligonucleotide sequences using an automated oligonucleotide synthesizer. The CS derivatives bearing a cyclic cis-1,2-diol exhibited efficient release of the two oligonucleotides under commonly employed basic conditions of aqueous ammonia. Among the CS analogues, it was found that CS with a robust structure can potentially be applied as a spacer molecule in the tandem synthesis of multiple oligonucleotides in a single sequence.