THE ANGIOGENIC POTENTIALS OF THE CEPHALIC MESODERM AND THE ORIGIN OF BRAIN AND HEAD BLOOD-VESSELS
THE ANGIOGENIC POTENTIALS OF THE CEPHALIC MESODERM AND THE ORIGIN OF BRAIN AND HEAD BLOOD-VESSELS
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DOI：
10.1016/0925-4773(95)00428-9
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发表时间：
1995-09-01
影响因子：
2.6
通讯作者：
LEDOUARIN, NM
中科院分区：
文献类型：
--
作者：
COULY, G;COLTEY, P;LEDOUARIN, NM
我们用两种分子标记物标记了早期鸟类胚胎血管内皮细胞及其前体细胞。一种标记物称为Quek 1，是哺乳动物VEGF受体flk-1的禽类同源物，另一种是MB 1/QH 1单克隆抗体。Quek 1在原肠胚形成阶段的中胚层细胞亚群中表达。Quek 1阳性细胞随后形成血管内皮细胞并表达MB 1/QH 1抗原，该抗原对鹌鹑物种的内皮细胞和造血细胞具有特异性。这两个标志物使我们首先表明，头部近轴中胚层具有血管生成的潜力，这是更广泛的比它的躯干对应物（体节）。其次，起源的内皮细胞内衬颅面和头部血管映射上的3体节阶段头的中胚层通过鹌鹑-鸡嵌合体技术，其中定义明确的中胚层领土之间交换阶段匹配的两个物种的胚胎在一个严格的同位素方式。我们发现，头部轴旁中胚层的前部区域主要是招募前脑和上面部的血管。这意味着大量的组织由头部中胚层的离散区域血管化，否则其命运是产生肌肉。由前近轴中胚层产生的血管生成细胞的广泛扩张必须与神经原基前部区域的高生长速率相关，从而产生端脑和神经嵴衍生的面部结构，这些结构本身缺乏血管生成能力。相比之下，在更后部的中胚层区域，血管生成细胞使大脑的相应水平血管化，它们另外产生的组织（骨骼和肌肉），以及下鳃区域的神经嵴衍生物。宿主和供体的成血管细胞的混合基本上限于如此限定的区域的边缘区，表明每个中胚层区域的内皮细胞根据受限且明确限定的模式使组织血管化。
We have used two molecular markers to label blood vessel endothelial cells and their precursors in the early avian embryo. One marker, called Quek1, is the avian homologue of the mammalian VEGF receptor flk-1 and the other is the MB1/QH1 monoclonal antibody. Quek1 is expressed in a subset of mesodermal cells from the gastrulation stage. Quek1 positive cells later form blood vessel endothelial cells and express the MB1/QH1 antigen which is specific for endothelial and hemopoietic cells of the quail species. These two markers allowed us first to show that the cephalic paraxial mesoderm has angiogenic potentials which are much more extended than its trunk counterpart (the somites). Secondly, the origin of the endothelial cells lining the craniofacial and head blood vessels was mapped on the 3-somite stage cephalic mesoderm via the quail-chick chimera technique, in which well defined mesodermal territories are exchanged between stage-matched embryos of both species in a strictly isotopic manner. We found that the anterior region of the cephalic paraxial mesoderm is largely recruited to provide the forebrain and the upper face with their vasculature. This means that large volumes of tissues are vascularized by a discrete region of the cephalic mesoderm, the fate of which is otherwise to give rise to muscles. The widespread expansion of the angiogenic cells arising from the anterior paraxial mesoderm must be related to the high growth rate of the anterior region of the neural primordium, yielding the telencephalon and of the neural crest-derived facial structures which are themselves devoid of angiogenic potencies. In contrast, in the more posterior mesodermal territories, the angiogenic cells vascularize the corresponding levels of the brain, the tissues (bones and muscles) that they otherwise produce, as well as the neural crest derivatives of the hypobranchial region. Mixing of angioblasts of host and donor is essentially restricted to the marginal zones of the territory so defined, showing that endothelial cells of each mesodermal region vascularize tissues according to a restricted and well defined pattern.