Production of 1,2-propanediol in photoautotrophic Synechocystis is linked to glycogen turn-over
Production of 1,2-propanediol in photoautotrophic Synechocystis is linked to glycogen turn-over
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DOI：
10.1002/bit.26468
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发表时间：
2018-02-01
影响因子：
3.8
通讯作者：
Buehler, Katja
中科院分区：
文献类型：
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作者：
David, Christian;Schmid, Andreas;Buehler, Katja
我们利用光自养生物以光为燃料，从二氧化碳和水合成 1,2-丙二醇。将包含 mgsA（甲基乙二醛合酶）、yqhD（醛还原酶）和 adh（乙醇脱氢酶）的合成途径插入集胞藻中。 PCC6803 将磷酸二羟基丙酮转化为甲基乙二醛，随后还原为丙酮醇，然后还原为 1,2-丙二醇。集胞藻可以成功生产1,2-丙二醇，产量约为55molh(-1)g(CDW)(-1)。令人惊讶的是，在稳定阶段观察到最大生产力。 1,2-丙二醇的产生明显与细胞内糖原的周转相关。当糖原池耗尽时，产物形成停止。减少糖原的碳通量显着降低了最终产物的滴度。培养条件的优化使得最终产物滴度接近1gL(-1) (12mM)，这属于迄今为止公布的该化合物光合自养生产的最高值。
We utilized a photoautotrophic organism to synthesize 1,2-propanediol from carbon dioxide and water fueled by light. A synthetic pathway comprising mgsA (methylglyoxal synthase), yqhD (aldehyde reductase), and adh (alcohol dehydrogenase) was inserted into Synechocystis sp. PCC6803 to convert dihydroxyacetone phosphate to methylglyoxal, which is subsequently reduced to acetol and then to 1,2-propanediol. 1,2-propanediol could be successfully produced by Synechocystis, at an approximate rate of 55molh(-1)g(CDW)(-1). Surprisingly, maximal productivity was observed in the stationary phase. The production of 1,2-propanediol was clearly coupled to the turn-over of intracellular glycogen. Upon depletion of the glycogen pool, product formation stopped. Reducing the carbon flux to glycogen significantly decreased final product titers. Optimization of cultivation conditions allowed final product titers of almost 1gL(-1) (12mM), which belongs to the highest values published so far for photoautotrophic production of this compound.