The promiscuity of immunoglobulin E binding to peanut allergens, as determined by Western blotting, correlates with the severity of clinical symptoms
The promiscuity of immunoglobulin E binding to peanut allergens, as determined by Western blotting, correlates with the severity of clinical symptoms
复制标题
DOI：
10.1111/j.1365-2222.2005.02252.x
复制
发表时间：
2005-06-01
影响因子：
6.1
通讯作者：
Hourihane, JOB
中科院分区：
文献类型：
--
作者：
Lewis, SA;Grimshaw, KEC;Hourihane, JOB
IgE 与特定蛋白质的结合已被证明与榛子的严重过敏反应有关。然而，IgE与特定花生过敏原的结合与症状严重程度之间的关系目前尚不清楚。为了确定IgE与特定花生过敏原的结合模式是否与临床症状的严重程度相关。40名花生过敏患者接受了双盲安慰剂对照的低剂量花生激发试验，在此期间对患者花生过敏的严重程度进行评分。测量了血清花生特异性 IgE (psIgE)，并分析了 IgE 与花生蛋白的结合模式。在 5 至 100 kDa 之间鉴定了 17 个 IgE 结合带，其中 8 个结合率超过 50% 的患者。每个患者的条带总数与挑战评分 (P=0.001, r=0.505) 和 psIgE (P < 0.001, r=0.820) 显着相关。聚类分析未能揭示特定蛋白质或蛋白质模式（基于存在/不存在）与挑战分数之间的任何关联。然而，两条蛋白质带（类似于 43 和 41 kDa）的峰值强度与挑战分数呈正相关，而第三条带（类似于 48 kDa）则呈负相关。这些条带分别被鉴定为 Ara h 3/4 和 1 的亚基。IgE 结合的混杂性似乎比单个蛋白质的识别更重要。这可能意味着如果仅使用选定的过敏蛋白，临床上有用的花生过敏特异性免疫疗法将难以实现。这项研究还强调了对 Ara h 1 和 3/4 亚基的进一步研究以及与症状严重程度的可能关联。
IgE binding to a specific protein has been shown to be associated with severe anaphylaxis to hazelnuts; however, the relationship between IgE binding to specific peanut allergens and symptom severity is currently unclear.To determine if the pattern of IgE binding to specific peanut allergens is associated with the severity of clinical symptoms.Forty peanut allergic patients underwent a double-blind placebo-controlled low-dose peanut challenge, during which the severity of the patients' peanut allergy was scored. Serum peanut-specific IgE (psIgE) was measured and IgE binding patterns to peanut proteins analysed.Seventeen IgE binding bands were identified between 5 and 100 kDa with eight bound by > 50% of patients. The total number of bands per patient correlated significantly with challenge score (P=0.001, r=0.505) and psIgE (P < 0.001, r=0.820). Cluster analysis failed to reveal any association between a particular protein or pattern of proteins (based on presence/absence) and challenge score. However, two protein bands (similar to 43 and 41 kDa) had peak intensities that correlated positively with challenge score and a third band (similar to 48 kDa) that correlated negatively. The bands were identified as subunits of Ara h 3/4 and 1, respectively.Promiscuity of IgE binding appears more important than the recognition of individual proteins. This may mean that clinically useful specific immunotherapy for peanut allergy will be difficult to achieve if only selected allergenic proteins are used. Further investigation of Ara h 1 and 3/4 subunits and a possible association with symptom severity are also highlighted by this study.